Homologs of the glutathione S-transferase (GST)-encoding gene were identified in a collection of aromatic hydrocarbon-degrading Sphingomonas spp. isolated from New Zealand, Antarctica, and the United States by using PCR primers designed from the GST-encoding gene of Sphingomonas paucimobilis EPA505. Sequence analysis of PCR fragments generated from these isolates and of the GST gene amplified f...

There has been growing interest in employing electro-bioremediation, a hybrid technology of bioremediation and electrokinetics for the treatment of contaminated soil. Knowledge however on the effect of weak electrokinetic conditions on the activity and viability of pollutant-degrading microorganisms is scarce. Here we present data about the influence of direct current (DC) on the membrane integ...

A variety of factors, including concentration of oil, antibiotics, dyes, and inoculum washes, were examined to determine their effect on the total counts of microorganisms on oil-containing media. The media found to be best for enumerating petroleum-degrading microorganisms contained 0.5% (vol/vol) oil and 0.003% phenol red, with Fungizone added for isolating bacteria and streptomycin and tetra...

Biodegradation of pyrene and indeno(1,2,3-cd)pyrene in reed rhizosphere soil was carried out in a small-scale wetland simulator using reed as well as microorganisms immobilized on cinder beads under natural environmental conditions. The results show that the stability of the introduced strains was enhanced upon immobilization and that the depletion of pyrene and indeno(1,2,3-cd)pyrene after 40 ...

Polycyclic aromatic hydrocarbons (PAHs) are chemically recalcitrant carcinogenic and mutagenic compounds with primarily anthropogenic origin. The investigation of the effects emissions from energy enterprises on soil microbiomes is a high priority for modern science. In this study, metagenomic profiling technogenic contaminated soils was carried out based bioinformatic analysis shotgun metageno...

This study evaluates the ability of two bacterial consortia (C2PL05 and BOS08), extracted from very different environments, to degrade low- (naphthalene, phenanthrene, anthracene) and high- (pyrene, perylene) molecular-weight polycyclic aromatic hydrocarbons (PAHs) at high (15-25 °C) and low (5-15 °C) temperature ranges. C2PL05 was isolated from a soil in an area chronically and heavily contami...

Cellulose-degrading microorganisms involved in the breakdown of plant cell wall material in the human gut remain rather unexplored despite their role in intestinal fermentation. Microcrystalline cellulose-degrading bacteria were previously identified in faeces of methane-excreting individuals, whereas these microorganisms were undetectable in faecal samples from non-methane excretors. This sugg...

Endophytes are ubiquitous in plants, and they may have a natural capacity to biodegrade polycyclic aromatic hydrocarbons (PAHs). In our study, a phenanthrene-degrading endophytic Paenibacillus sp. PHE-3 was isolated from P. asiatica L. grown in a PAH-contaminated site. The effects of environmental variables on phenanthrene biodegradation by strain PHE-3 were studied, and the ability of strain P...