P pili are important adhesive fibres that are assembled by the conserved chaperone-usher pathway. During pilus assembly, the subunits are incorporated into the growing fibre by the donor-strand exchange mechanism, whereby the beta-strand of the chaperone, which complements the incomplete immunoglobulin fold of each subunit, is displaced by the amino-terminal extension of an incoming subunit in ...

An in-house loop-mediated isothermal amplification (LAMP) reaction was established and evaluated for sensitivity and specificity in detecting the presence of Salmonella Typhi (S. Typhi) isolates from Kelantan, Malaysia. Three sets of primers consisting of two outer and 4 inner were designed based on locus STBHUCCB_38510 of chaperone PapD of S. Typhi genes. The reaction was optimised using genom...

A fundamental question in molecular biology is how proteins fold into domains that can serve as assembly modules for building up large macromolecular structures. The biogenesis of pili on the surface of Gram-negative bacteria requires the orchestration of a complex process that includes protein synthesis, folding via small chaperones, secretion, and assembly. The results presented here support ...

BACKGROUND The effectiveness of strategies for treatment of the altered static lung volume and against the development of bronchial hyperreactivity (BHR) following a left ventricular dysfunction (LVD) induced by myocardial ischaemia was investigated in a rat model of sustained postcapillary pulmonary hypertension. METHODS Airway resistance (Raw) was identified from the respiratory system inpu...

In this paper, a decentralized asymptotic fault tolerant control system is proposed for near space vehicle (NSV) attitude dynamics. First, NSV reentry mode is described, and the actuator failure model is developed whose behavior is described by high-order dynamics. Next, the multi-model based fault diagnosis and identification (FDI) algorithm is proposed for high order actuator dynamics, which ...

The present investigation aims to the isolation of Streptomycetes from saline soil. The isolates were varied in color to aerial vegetative mycelia, morphology of conidospore surface, melanin production, utilization of carbon; nitrogen source and fatty acid analysis, as well as physical and chemical characters for their habitat soil. Streptomycetes isolates exhibiting wide range of variability. ...

Many applications involve monitoring incidence rates of the Poisson distribution when sample size varies over time. Recently, a couple of cumulative sum and exponentially weighted moving average (EWMA) control charts have been proposed to tackle this problem by taking the varying sample size into consideration. However, we argue that some of these charts, which perform quite well in terms of av...