Pasteuria penetrans isolate P-20 has been attributed as the cause of soil suppressiveness to peanut root-knot nematode in Florida. In this study, P. penetrans was transferred from a suppressive site to a new site and established by growing susceptible hosts to the peanut root-knot nematode during both summer and winter seasons. When two soil fumigants, 1,3-dichloropropene (1,3-D) and chloropicr...

Pasteuria penetrans spore adhesion to Meloidogyne javanica second-stage juveniles (J2) was examined following several different pretreatments of the latter. The detergents sodium dodecyl sulfate and Triton X-100, the carbohydrates fucose and alpha-methyl-D-mannoside, and the lectins concanavalin A and wheat germ agglutinin reduced spore attachment. Spores exposed to M. javanica surface coat (SC...

A 3-year study was conducted to evaluate fenamiphos at 20.2 kg a.i./ha applied in both fall and spring or in spring only for the control of Pratylenchus penetrans on apple, Malus domestica cv. Granny Smith on M7a rootstock. The initial population densities of P. penetrans within the plot area were 89/250 cm(3) soil and 268/g root dry weight. Fenamiphos increased (P < 0.05) trunk diameter in yea...

Pasteuria penetrans is an endospore-forming bacterial parasite of root-knot nematodes that has potential as a biological control agent. Biochemical investigations of P. penetrans are limited because of difficulty in obtaining large quantities of endospores free of plant debris and contaminating microorganisms. Our objective was to develop a technique for extraction and purification of P. penetr...

Pasteuria penetrans Sayre & Starr parasitizes several nematode species and is considered a promising biocontrol agent of the root-knot nematode, Meloidogyne spp. (1,7). Numerous plant-parasitic nematodes, including Tylenchulus semipenetrans, however, are seldom if ever infected by this biocontrol agent (3). Tylenchulus semipenetrans Cobb is the most economically important and widespread nematod...

&lt;p&gt;&lt;strong&gt;Background&lt;/strong&gt;: &lt;em&gt;Pasteuria penetrans&lt;/em&gt; is a nonculturable bacterium that obligately parasitizes several species of phytopathogenic nematodes. Unknown endogenous female, plant or microbial reproductive factors are indispensable for multiplication and endospore formation &lt;em&gt;P. penetrans&lt;/em&gt;. &lt;strong&gt;Objective&lt;/strong&gt;: ...

A simple method for atomic force microscopy (AFM) of nematode cuticle was developed to visualize the external topography of Helicotylenchus lobus, Meloidogyne javanica, M. incognita, and Xiphinema diversicaudatum. Endospores of two isolates of the nematode parasite, Pasteuria penetrans, adhering to M. incognita and X. diversicaudatum were also visualized and measured by this technique. Scanning...

Host-parasite relationships of Meloidogyne incognita acrita and Pratylenchus penetrans were compared on three closely related cultivars of tomato: 'Nemared', resistant to root-knot nematodes; 'Hawaii 7153', moderately resistant; and 'B-5', susceptible. Root-knot nematode larvae induced typical galls on the roots of B-5; larvae that entered Nemared were walled-off by necrotic cells; both reactio...

The synthesis and localization of an endospore surface epitope associated with the development of Pasteuria penetrans was determined using a monoclonal antibody (MAb) as a probe. Nematodes, uninfected or infected with P. penetrans, were harvested at 12, 16, 24, and 38 days after inoculation (DAI) and then examined to determine the developmental stage of the bacterium. Vegetative growth of P. pe...