In polymerase chain reaction (PCR)-based nucleic acid quantification, the DNA template type, primer/probe sequence, and instrument platform such as real-time quantitative PCR (qPCR) digital (dPCR) affect accuracy reliability of results. this study, a plasmid (pDNA) pBI121-screening, genetically modified (GM) rice SDrice genomic (gDNA), GM rapeseed SDrape gDNA, all carrying same 11 screening ele...

Glutamine synthetase được mã hóa bởi gen GS1 là enzyme đồng nitrogen, dùng amoniac như một cơ chất xúc tác cho phản ứng chuyển glutamic acid thành glutamine phụ thuộc vào ATP. Dựa trên cặp XmaI và SacI, chúng tôi đã thiết kế công cấu trúc mang (pBI121/GmPrP2:GS1_cmyc:NOS) biến nạp vi khuẩn Agrobacterium tumefaciens. Tiến hành thí nghiệm hơn 700 thể nhận các đoạn thân mầm cây in vitro, thu 33 ch...

In order to improve the salt tolerance of some canola genotypes to be suitable for cultivation in Kingdom of Saudi Arabia (KSA), the salt tolerance gene (mt1D) encoding for mannitol-1-phosphate dehydrogenase was introduced to the canola plants using the agrobacterium-mediated transformation. To achieve that, five canola genotypes were selected as a relatively salt tolerance among 15 canola geno...

Regeneration of in vitro multiple shoots was achieved through organogenesis on MS supplemented with 2.0 mg/l BAP and 0.5 Kn from cotyledonary leaf explants two local varieties eggplant (Solanum melongena L.). Elongation regenerated obtained growth regulator free MS. In root induction excised less effective or without plant regulators. On the other hand treated 10 mM IBA for 5 min were found to ...

cotton cultivar coker has been already transformed with recombinant pbi121-chi via agrobacterium tumefaciens. the t-dna region of pbi121-chi carries the chitinase (chi ) gene from bean and is under the control of the camv35s promoter. t1 and t2 progenies of transgenic cotton containing the chi gene were used in this study.  polymerase chain reaction (pcr), southern and western blotting data con...

A method for transformation of B.napus is presented in this study. The protocol is based on infection of cotyledonary petioles of 5-day-old and hypocotyl segments of 14-day-old seedlings with an Agrobacterium tumefaciens strain LBA 4404 carrying a disarmed binary vector pBI121. The T-DNA region of this binary vector contains the neomycin phosphotransferase II (NPTII) and glucuronidase (GUS) gen...