نتایج جستجو برای: pcr based marker
تعداد نتایج: 3177678 فیلتر نتایج به سال:
Custom genome editing has become an essential element of molecular biology. In particular, the generation of fusion constructs with epitope tags or fluorescent proteins at the genomic locus facilitates the analysis of protein expression, localization, and interaction partners at physiologic levels. Following up on our initial publication, we now describe a considerably simplified, more efficien...
The present study on SSR marker-based genetic diversity and marker-trait association analysis in aromatic rice (Oryza sativa L.) landraces was carried out at R. H. Richharia Research Laboratory, Indira Gandhi Krishi Vishwavidyalaya, Raipur, Chhattisgarh during 2020. A total of 25 PCR-based simple sequence repeats (SSR) markers were evaluated a set 90 along with 6 checks which includes 1 non-aro...
the study investigates the possibility of sex determination of rainbow trout (oncorhynchus mykiss)using molecular screen for products monosex of populations. 35 caudal fin tissue samples wereobtained from karaj rainbow trout mature population and then genomic dna was extracted andmarker loci amplified. polymerase chain reaction (pcr) was setup based on two omyfa andomyfatu markers for amplifica...
Sixty isolates of Pythium aphanidermatum as the causal agent of sugar beet root rot were selected on the basis of their geographical origins, morphological and genetic diversity studies. Pathogenicity test using the Kruskal-Wallis analysis showed significant differences between the pathogenic potential of isolates. Based on the growth rate, isolates were categorized in two groups with low and h...
BACKGROUND Sensitive, specific blood-based tests are difficult to develop unless steps are taken to maximize performance characteristics at every stage of marker discovery and development. We describe a sieving strategy for identifying high-performing marker assays that detect colorectal cancer (CRC)-specific methylated DNA in plasma. METHODS We first used restriction enzyme-based discovery m...
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