Design of primers for efficient DNA amplification using polymerase chain reaction is critical. Therefore, evaluation of hairpin structures while designing position dependent primers needs special attention. Here, we present the data on the effect of hairpins on DNA amplification using polymerase chain reaction. X-antigen of Hepatitis B Virus has been used as a model. Studies showed that hairpin...

fluorescent monitoring of dna amplification is the basis of real-time pcr. absolute quantification can be achieved using a standard curve method. the standard curve is constructed by amplifying known amounts of standards under identical conditions to that of the samples.the objective of the current study is to propose a mathematical model to assess the acceptability of pcr resulys.four commerci...

Our objective was to use long PCR to determine the copy number of ptDNA and mtDNA in total DNA extracted from tissue samples. In previous work, the efficiency of amplification of DNA decreased as the target sequence length increased above about 1 kb, so that DNA quantification using long PCR was considered unreliable (Arezi et al. 2003). Here, we used a long-PCR endpoint procedure to amplify or...