This study aims to create a quantitative polymerase chain reaction (q-PCR) methodology for monitoring the hydrogen-producing mixed cultures enriched from elephant dung using alpha-cellulose as carbon source through five generations of repetitive sub-culture. The thermophilic fifth cultivation cycle gave highest hydrogen yield 170.3 mL H2/g cellulose and were used generate sawdust. Clostridium s...

Abstract Background Patients with sterile pyuria may be infected sexually transmitted diseases or have renal tuberculosis. This study investigated the possibility of detecting and Mycobacterial tuberculosis in samples polymerase chain reaction. Methods Forty-four day-3 negative urine culture were for presence Results Among 44 samples, 61.4% positive by reaction (PCR) bacterial DNA (either Mycob...

A nested polymerase chain reaction (PCR) was developed to detect genomic DNA of Piscirickettsia salmonis, the causative agent of an epizootic disease in salmonids. The nested PCR assay, which used general bacterial 16s rDNA primers in the flrst amplification reaction, and P salmonisspecif~c primers in a second reaction, allowed detection of less than 1 P salmonis tissue culture infect~ous dose ...

Using Single Multiplex PCR Reaction to Identify Candida Species in Vaginal isolates: Comparison between Phenotypic and Molecular Methods

We describe a photochemical procedure for the sterilization of polynucleotides that are created by the Polymerase Chain Reaction (PCR). The procedure is based upon the blockage of Taq DNA polymerase when it encounters a photochemically modified base in a polynucleotide strand. We have discovered reagents that can be added to a PCR reaction mixture prior to amplification and tolerate the thermal...

background: newcastle disease is one of the most serious viral diseases in the poultry worldwide. objectives: since the traditional strategies have been hardly effective in controlling the disease, the purpose of this study was to introduce new methods for early and rapid diagnosis of newcastle. the present study helps to reduce further damage to the poultry industry. methods: rna extraction wa...

Abstract Additive manufacturing for microfluidics shows potential to boost research and development in biology molecular diagnostics. This paper reports on novel process material optimisation techniques the creation of a monolithic microfluidic chip geometry polymerase chain reaction (PCR) thermocycling using stereolithography (SLA). A two-stage printing protocol with projection SLA is assessed...

This study developed a new multiplex polymerase chain reaction (m-PCR) for rapidly detecting clinically essential strains of V. parahaemolyticus. enables the detection total and potentially virulent strains. The m-PCR was by targeting species-specific transcriptional regulator toxR gene, sequences an outer membrane protein hypothetical encoded omp htp, respectively. htp were discovered original...