Background/Aims Increased salivary pepsin could indicate an increase in gastro-esophageal reflux, however, previous studies failed to demonstrate a correlation between salivary pepsin concentrations and 24-hour esophageal acid exposure. This study aims to detect the salivary pepsin and to evaluate the relationship between salivary pepsin concentrations and intercellular spaces (IS) in different...

The pepsin used in the preliminary experiments of this study was prepared from Parke, Davis pepsin by the method of Philpot (2) and was twice recrystallized. It was stored until used under half saturated magnesium sulfate solution at 2”. Pepsin A was prepared from Cudahy pepsin by the fractionation procedure of Herriott, Desreux, and Northrop (3). Its solubility in 0.05 M acetate, pH 4.6, 0.5 s...

OBJECTIVES At the cellular level, tissue-bound pepsin is fundamental to the pathophysiologic mechanism of reflux disease, and although the thresholds for laryngeal damage in laryngopharyngeal reflux and for esophageal damage in gastroesophageal reflux disease differ, both forms of damage are due to pepsin, which requires acid for its activation. In addition, human pepsin remains stable at pH 7....

The conversion of pepsinogen to pepsin has been followed by means of the fluorescent probe, Z-p-toluidinonaphthalene6-sulfonate (TNS). It was found that the change in fluorescence accurately reflects the formation of active pepsin. The data on the binding of TNS to pepsinogen and pepsin suggest that the environment surrounding the fluorophore is altered during the conversion. One of the peptide...

The control of gastric pepsin secretion continues to excite controversy. The diversity in the results obtained by different investigators may be due, at least in part, to the varying techniques employed to estimate pepsin activity. Thus, edestin (Polland and Bloomfield, 1929), gelatin (Gilman and Cowgill, 1930), egg albumen (Vineberg and Babkin, 1931), beef haemoglobin (Anson and Mirsky, 1932),...

Crystalline pepsin has been acetylated by the action of ketene in aqueous solution at pH 4.07-5.5. As acetylation proceeds the activity decreases, the decrease being more rapid at pH 5.0-5.5 than at 4.0-4.5. Three acetyl derivatives have been isolated from the reaction mixture and obtained in crystalline form. The crystal form of these derivatives is similar to that of pepsin. Fractionation and...

A miniaturized pepsin reactor was prepared inside a fused-silica capillary (i.d. 75 microm) by coating a pepsin-containing gel on a photopolymerized porous silica monolith. The pepsin-encapsulated film was prepared by a sol-gel method. The sol-gel reaction was optimized so that the sol solution containing pepsin forms a thin film on the photopolymerized sol-gel (PSG) monolith that was initially...

Strong resistance to proteolytic attack is important for feed enzymes. Here, we selected three predicted pepsin cleavage sites, L99, L162, and E230 (numbering from the initiator M of premature proteins), in pepsin-sensitive HAP phytases YkAPPA from Yersinia kristensenii and YeAPPA from Y. enterocolitica, which corresponded to L99, V162, and D230 in pepsin-resistant YrAPPA from Y. rohdei. We con...