This study was undertaken to investigate the enzymatic regulation of the biosynthesis of vasoconstrictor prostanoids by resting and interleukin (IL)-1(beta)stimulated human umbilical vein endothelial cells (HUVECs). Biosynthesis of eicosanoids in response to IL-1beta, exogenous labeled arachidonic acid (AA), or histamine, as well as their spontaneous release, was evaluated by means of HPLC and ...

Diabetic endothelial dysfunction is characterized by impaired endothelium-dependent relaxation. In this study, we measured the expression of endothelial nitric oxide synthase (eNOS), cyclooxygenase-1 (COX-1), cyclooxygenase-2 (COX-2), prostacyclin synthase (PGIS), and prostacyclin receptor (IP) in subcutaneous arteries of type-2 diabetic and non-diabetic patients. Subcutaneous arteries were dis...

Prostaglandins (PGs) are typical lipid mediators that play a role in homeostasis and disease. They are synthesized from arachidonic acid by cyclooxygenase 1 (COX1) and COX2. Although COX2 has been reported to be upregulated in the spinal cord after nerve injury, its expression and functional roles in neuropathic pain remain unclear. In this study, we investigated the expression of Cox2, PGI2 sy...

We studied the arachidonic acid (AA)-mediated modulation of large-conductance Ca2+-activated K+ (BK) channels in coronary arterial smooth myocytes from lean control and Zucker Diabetic Fatty (ZDF) rats. A total of 1 micromol/l AA enhanced BK current by 274% in lean and by 98% in ZDF rats. After incubation with 10 micromol/l indomethacin, 1 micromol/l AA increased BK currents by 80% in lean and ...

For years, a GIS has been called a decision support system (Cowen 1988). When we connect GIS with participatory contexts we get “participatory GIS,” (PGIS) (Harris et al. 1995, Jankowski and Nyerges 2001) or a “public participation GIS” (PPGIS) (Nyerges, Barndt, Brooks 1997), or community integrated GIS (Craig, Harris, and Weiner 2001). The term public is defined by the American Heritage Dictio...

Effective risk management and control methods for potentially genotoxic impurities (PGIs), including alkyl halides, are of significant importance in the medicinal (pharmaceutical) sector. The three halides posaconazole PGIs. detection assessment substances is a top priority all regulatory organizations. Quantifying PGIs at trace levels using standard analytical techniques, such as gas chromatog...

Abstract Objectives To measure in vivo 3D accuracy of backward‐planned partially guided implant surgery (PGIS) based on dental magnetic resonance imaging (dMRI). Material and Methods Thirty‐four patients underwent dMRI examinations. Tooth‐supported templates were backward planned using standard software, 3D‐printed, placed intraorally during a cone beam computed tomography (CBCT) scan. Treatmen...

In obesity, skeletal muscle blood flow during exercise (functional hyperemia) is impaired. We have indirectly demonstrated that an altered arachidonic acid metabolism is responsible for the impaired functional vasodilation in the obese Zucker rat (OZR), a model of obesity. In this study, we tested the hypothesis that there is an impaired release of PGI(2) due to a nitration of PGI(2) synthase (...

The aim of this paper is to understand the social use and the consequences of PGIS in local communities, more specifically in Sierra Nevada, Mexico. In order to investigate the use of ICT by communities, we applied a multilevel and structurationist framework that articulates three dimensions: context, process and content. The results of our study draw attention to the implications of using PGIS...