Continuous monitoring of the pH of tissue with a glass electrode was initially introduced by Stamm et al. (1). Its potential value in monitoring the high-risk fetus during labor has been demonstrated in several studies (2-4). However, this technique has not been clinically accepted for routine use in labor, mostly because of problems with the application and sustained proper attachment of the r...

Luminal pH is an important functional feature of intracellular organelles. Acidification of the lumen of organelles such as endosomes, lysosomes, and the Golgi apparatus plays a critical role in fundamental cellular processes. As such, measurement of the luminal pH of these organelles has relevance to both basic research and translational research. At the same time, accurate measurement of intr...

Accurate measurement of pH in concentrated brines is a critical aspect of materials testing in the oil and gas industry. Concentrated sodium chloride solutions, typically up to 4 M and saturated with carbon dioxide, are often used to simulate production water during testing programmes. The pH of the solution may be adjusted to the desired value by the addition of sodium bicarbonate (to raise pH...

In this article, we describe the advantages and disadvantages of procedures for monitoring mitochondrial pH in situ using optical microscopic techniques. The first method employs the combination of the fluorescent pH-sensitive indicator carboxy-SNARF and laser scanning confocal microscopy. Manipulation of the loading and post-loading conditions enables relatively specific accumulation of carbox...

Vaginal pH measurement is a screening method of vaginal infection in pregnancy. We tested two different methods of pH determination. In freshly taken vaginal fluid measurements were carried out with special pH indicator strips and pH micro glass electrode. About 100 microliters of vaginal fluid is enough when pH indicator strips are used, and 200 microliters when the micro electrode is used. Th...

Background: The RightLevelpHTM indicator is a new device designed to measure pH of gastric aspirate obtained via nasogastric tube while minimizing exposure of the operator. Study Objectives: In vivo and in vitro validation of the RightLevelpHTM indicator. Methods: Patients (23) undergoing general anesthesia had placement of a nasogastric tube and gastric pH electrode catheter following endotrac...

We used three methods to examine the relationship among intracellular pH, transmembrane potential, and extracellular pH. Single-barreled electrodes permitted the determination of resting potential and intracellular pH with a minimum of cellular injury. Double-barreled electrodes, which incorporated a reference as well as a pH-sensitive electrode in a single tip, facilitated the direct measureme...

When crops do not grow well, one of the first question an agronomist is likely to ask is “What is the pH of the soil?” The reason for this question is that soil pH tells us more about a soils ability to produce crops that any other single measurement. A measurement of soil pH is like a doctor’s measurement of a patient’s temperature. It gives an indication something may or may not be wrong, but...