Introduction Chromatography is an effective and very useful method for separation and purification of organic compounds that can be used even for complex mixtures. Chromatography separates components of a mixture based upon how well they are adsorbed on an inert phase (the stationary phase) versus how well they dissolve in a liquid phase (the mobile phase) (Figure 1). A mixture is introduced t...

Introduction Chromatography is an effective and very useful method for separation and purification of organic compounds that can be used even for complex mixtures. Chromatography separates components of a mixture based upon how well they are adsorbed on an inert phase (the stationary phase) versus how well they dissolve in a liquid phase (the mobile phase) (Figure 1). A mixture is introduced t...

Abstract The application of temperature gradient interaction chromatography (TGIC) as an advanced technique for the characterisation polymers is discussed, in comparison to other liquid techniques and particular ubiquitous size exclusion chromatography. Specifically, use reversed-phase TGIC interrogation complex branched normal-phase high-molar mass end-functionalised highlighted.

An optimization strategy for ternary solvent-strength gradient elution RP chromatography is described in which a two-dimensional model of gradient time (2 levels) against ternary proportions of organic modifiers (4 levels) was constructed. From the resolution surface the optimum ratio of organic modifiers could be selected. Excellent retention time and acceptable peak width and resolution simul...