aflatoxins are a group of fungal toxic metabolites, which are contaminated certain food commodities. elisa is one of the sensitive methods for detection of aflatoxins. preparation and stabilizing of a proper conjugated tracer for detection of aflatoxins is probably the main step for designing an elisa method. in current study, different stabilizers were applied to stabilize a newly prepared con...

We evaluated a newly developed solid-phase immunoassay (EIA) of terminal deoxynucleotidyl transferase (TdT, EC 2.7.7.3) and compared it with the enzymatic assay of TdT involving DNA polymerase. We assessed the precision, performance characteristics, and clinical efficacy of the EIA procedure, using 249 specimens of peripheral blood and bone marrow and 118 specimens of whole blood. On linear reg...

A solid-phase enzyme immunoassay is described for measuring hepatitis B surface antigen in human serum or plasma. Immunologically purified antibody labeled with horseradish peroxidase was used as the indicator. In the assay system, antibody-coated controlled-pore glass is used as a solid support and there are three sequential incubations, totaling 2 h, at room temperature. Results for serially...

Using two monoclonal antibodies directed against urokinase, we have developed a micro enzyme-linked immunosorbant assay (ELISA) to detect and measure urokinase in biological fluids. The system presents the following characteristics: simple and rapid procedure, reproducibility, sensitivity (urokinase levels down to 1 ng/ml) and evaluation of the enzyme in biological fluids such as urine, pleural...

We have developed a rapid enzyme immunoassay for progesterone in saliva. This solid-phase assay is carried out on microtitre plates with no extraction or centrifugation steps. The detection limit of the assay is 200 fg per well (3.2 pmol/L). Intra- and interassay coefficients of variation for low, medium, and high concentrations of progesterone were 7.5, 16.0; 9.1, 8.3; and 8.7, 6.7%, respectiv...

A simple, direct chemiluminescence immunoassay for progesterone in mixed, unstimulated saliva is described. We use purified polyclonal anti-progesterone antibodies covalently coupled to polyacrylamide beads and progesterone-11 alpha-hemisuccinyl-aminobutylethyl isoluminol as the chemiluminescent ligand marker. Bound and free ligand are separated by simple centrifugation. The detection limit of ...

an aid in the diagnosis of prostate cancer since 1936 when Gutman et a!. (13) first reported the relationship between serum acid phosphatase and prostate cancer. They found that serum acid phosphatase activity markedly increased in patients with prostate carcinoma, especially in those with bone metastasis. Elevated serum acid phosphatase activity has been reported in 70 to 90% of the patients w...

We describe an enzyme immunoassay for human serum ferritin in which antibody adsorbed on polystyrene tubes is used. Adsorbed gamma-globulins against human ferritin were first allowed to react with ferritin and a second antiferritin antibody, labeled with alkaline phosphatase, was added. The amount of bound enzyme/antibody conjugate was proportional to the ferritin titer in the assay. This metho...

We describe a time-resolved fluoroimmunoassay for the cardiac glycoside digoxin. The assay depends on the competitive distribution of Eu3+-labeled anti-digoxin antibodies between solid-phase-bound digoxin and the digoxin in the sample or standard. After this immunoreaction, the bound fraction of the Eu3+-label is dissociated from the solid phase, converted into a highly fluorescent beta-diketon...

We describe a simple, solid-phase chemiluminescence immunoassay for progesterone in 10 microL of unextracted serum ("direct" assay). Danazol at pH 8.0 is included (100 ng per tube) to displace progesterone from binding proteins in serum. A progesterone-11 alpha-hemisuccinyl-aminobutylethyl isoluminol conjugate serves as the chemiluminescent ligand marker and homologous antiprogesterone IgG cova...