Theobroma cacao is a woody and recalcitrant plant with a very high level of interfering compounds. Standard protocols for protein extraction were proposed for various types of samples, but the presence of interfering compounds in many samples prevented the isolation of proteins suitable for two-dimensional gel electrophoresis (2-DE). An efficient method to extract root proteins for 2-DE was est...

Isolation of high-quality RNA of Avicennia germinans L. tissue is difficult due to high levels of phenols and other substances that interfere when using conventional procedures for the isolation. These substances not only decrease the yield but also the quality of RNA is almost poor. We present here a simple RNA protocol and fast methodology that effectively removes these contaminating substanc...

Twenty six accessions of grain Amaranth (Amaranthus hypochondriacus L.) were evaluated for salient biochemical and quantitative traits particularly reference to chlorophyll a and b, total chlorophyll, phenol content, leaf moisture, leaf protein content, test weight and yield plant. Genetic divergence and association among these traits were analysed. Chlorophyll a, chlorophyll b, total chlorophy...

Pyrolysis of palm kernel shell in a fixed-bed reactor was studied in this paper. The objectives were to investigate the effect of pyrolysis temperature and particle size on the products yield and to characterize the bio-oil product. In order to get the optimum pyrolysis parameters on bio-oil yield, temperatures of 350, 400, 450, 500 and 550 °C and particle sizes of 212–300 μm, 300–600 μm, 600μm...

INCE the very early experiments of GRAUBARD (1933), numerous investigators have examined the problem of genetic control of phenol oxidases in Drosophila with attention focused primarily on pigment production and thus on the color mutants. However, it has become clear that the function of such enzymes in cuticle formation is far more vital than in pigment production. It follows that if the same ...

Guarana has great agricultural potential and is largely used therapeutically and in the production of non-alcoholic energy drinks. Genomic and proteomic studies are crucial to identify proteins that play central roles in the maintenance and viability of fruits, as well as to identify proteins related to the main metabolic pathways. However, the success of any protein analysis starts with the pr...

Plant tissues contain relatively low amounts of proteins whose extraction is often difficult due to the presence of interfering compounds such as rigid cellulosic cell wall, storage polysaccharides, lipids and other contaminants that can cause protein degradation or modification. Therefore it is important to optimize protein extraction and to establish a robust protocol for two-dimensional gel ...

A new technique was proposed to co-produce phenol and activated carbon (AC) from catalytic fast pyrolysis of biomass impregnated with K3PO4 in a hydrogen atmosphere, followed by activation of the pyrolytic solid residues. Lab-scale catalytic fast pyrolysis experiments were performed to quantitatively determine the pyrolytic product distribution, as well as to investigate the effects of several ...

The genetic accessibility of selected acidophilic bacteria was investigated to evaluate their applicability to degrading pollutants in acidic environments. The IncP1 antibiotic resistance plasmids RP4 and pVK101 and the phenol degradation-encoding plasmid pPGH11 were transferred from neutrophilic bacteria into the extreme acidophilic eubacterium Acidiphilium cryptum at frequencies of 1.8 x 10(-...

Synthesis of complex tetraoxo-μ-oxobispentamethylpentacyclodienyl-dimolybdenum, Cp*2Mo2O5 was carried out in THF using Mo(CO)6 and NaCp* as starting materials. Oxidizing the intermediate, Na[Cp*Mo(CO)3], with 6 equivalent of TBHP under basic condition follow by acidification in aqueous resulting the product, Cp*2Mo2O5, was obtained in reasonable yield (42%). Characterizations of the synthesized...