Polyprenyl phosphates, as well as polyprenyl alcohols bearing different isopentenyl C(5) units, have been synthesized. The pH range of spontaneous vesicle formation of polyprenyl phosphates with or without polyprenyl alcohols was defined by fluorescence microscopy. A variety of the acyclic or monocyclic polyprenyl phosphates studied formed stable vesicles in water over a wide range of pHs, and ...

The interest in electrocrystallization of calcium phosphates (CaP) in general, and of hydroxyapatite (HAp) specifically, results from the promising industrial benefits, unique microstructures, and properties of the deposits produced by this process, and the possible similarity to bone mineralization in vivo. In this paper, our work on electrocrystallization of HAp and octacalcium phosphate (OCP...

Coupled enzyme assays are described for measuring inorganic phosphates, organic phosphates and phosphate-liberating enzymes in biological material. The assays all determine Pi by its reaction with inosine, catalysed by nucleoside phosphorylase; this yields ribose 1-phosphate and hypoxanthine. The hypoxanthine is oxidized to uric acid by xanthine oxidase, and may be measured either by the absorb...

Bisphosphonates (BPs) are the most utilized drugs for the treatment of osteoporosis, and are usefully employed also for other pathologies characterized by abnormally high bone resorption, including bone metastases. Due to the great affinity of these drugs for calcium ions, calcium phosphates are ideal delivery systems for local administration of BPs to bone, which is aimed to avoid/limit the un...

The present study deals with the effect of increased concentrations of alcohols (methanol and ethanol) on the solubilization of FePO4 and CaHPO4 by Aspergillus niger. The efficiency of solubilization (ES) of inorganic phosphates, fungal growth, acid production and pH variation were determined. The concentrations of alcohols that most favored the solubilization of phosphates were 4% methanol and...

A HPLC method was developed for separation and quantitative determination of inositol tri-, tetra-, penta-, and hexaphosphates. The method included extraction of inositol phosphates with HCl, separation of the inositol phosphates from the crude extract by ion-exchange chromatography, and ion-pair Cl8 reverse phase HPLC analysis using formic acid/methanol and tetrabutylammonium hydroxide in the ...

Katheryn Adam, Chemistry Faculty Mentor: Marco Bonizzoni, Chemistry An off-the-shelf sensing system for physiologically relevant phosphates We have developed a chemical sensing system that can differentiate biologically relevant phosphates (nucleoside diphosphates, pyrophosphate) in neutral water solution using only commercially available components. Our approach uses a common fluorescent indic...

Recent studies have considerably clarified the mechanism ofhydrolysis of ribonucleic acids (RNA) by pancreatic ribonuclease and by spleen phosphodiesterase. Digestion ofRNA with ribonuclease results in the splitting of all pyrimidine-pyrimidine internucleotidic links and of those pyrimidine-purine internucleotidic links which involve C(3w) of the pyrimidine nucleotide (Schmidt et al. 1951; Mark...