When (32)P(1) was supplied as a 15-minute pulse to normal Spirodela oligorrhiza plants, the first phospholipid to become fully labeled was phosphatidic acid. Phosphatidyl glycerol reached maximum labeling before the other major phospholipids. In phosphorus-deficient plants, however, phosphatidyl glycerol became labeled much more slowly than either phosphatidyl choline or phosphatidyl ethanolami...

We have monitored the plasma concentrations of products of the transsulfuration pathway in 11 undernourished noncirrhotic patients, and in 10 cachectic cirrhotic subjects, before and during nasoenteral nutrition with Vivonex (Norwich-Eaton Pharmaceuticals, Norwich, NY) or total parenteral nutrition (TPN) with FreAmine III (American McGaw, Irvine, CA). In the cirrhotic cases eating a mixed diet,...

Membranous material was prepared from Ehrlich ascites carcinoma cells by tonal centrifugation. The distribution of fatty acids among the individual neutral lipid and phospholipid classes isolated from the membrane preparation was determined. Survey electron microscopy indicated that the preparations consisted of vesicles of rough surfaced endoplasmic reticulum, lysosomal-like structures, virus-...

BACKGROUND Long chain polyunsaturated fatty acids (LCPUFAs) including docosahexaenoic acid and arachidonic acid are suspected to play a key role in the pathogenesis of diabetes. LCPUFAs are known to be preferentially concentrated in specific phospholipids termed as plasmalogens. This study was aimed to highlight potential changes in the metabolism of phospholipids, and particularly plasmalogens...

Glyoxysome, endoplasmic reticulum, mitochondria, and proplastid fractions were isolated from endosperm of castor beans (Ricinus communis) germinated for 5 days at 30 C. Samples from sucrose density gradients were diluted with 0.15 m KCI and the membranes pelleted. Lipid extracts of these membranes were analyzed for phosphoglyceride, acyl lipid, and sterol content. The endoplasmic reticulum cont...

Chloroform-methanol 2:1 removes a significantly greater quantity of lipid from bleached bovine retinal rods than from a dark-adapted counterpart. The extracts contain phosphatidyl serine, phosphatidyl choline, phosphatidyl ethanolamine, sphingomyelin, and an unknown substance which, it is proposed, may be a combination of phospholipid and retinaldehyde. The difference between extracts of light-...

the effects of camp-analogue dibutyryl-camp and anticalmodulin w-7 were studied on de novo synthesis and secretion of lipids in cultures of hepatoma mcardle rh7777 cells and normal rat hepatocytes. dibutyryl-camp and w -7 separately caused a significant decrease in the secretion of de novo synthesized triacyl [3h]glycerol in both cultures of mcardle cells and rat hepatocytes. the inhibitory eff...

Fatty acid methyl esters and dimethylacetals suitable for gas chromatographic analysis were prepared by treatment of lipids with boron fluoride-methanol (140 g BFI per liter of methanol). This reagent is stable and easy to handle. Reaction conditions were investigated for triglycerides, diglycerides, monoglycerides, free fatty acids, sterol esters, phosphatidyl ethanolamines, phosphatidyl serin...

The preservation for electron microscopy of saturated phospholipids in general, and phosphatidyl choline (PC)in particular, remains and unsolved problem since OsO(4) and glutaraldehyde are incapable of interacting with PC directly. However, by introducing tannic acid preceding osmication, we were able to demonstrate highly ordered, preserved lamellar structures in model experiments with saturat...