BACKGROUND Phytophthora is the most important genus of the Oomycete plant pathogens. Nowadays, there are 117 described species in this genus, most of them being primary invaders of plant tissues. The different species are causal agents of diseases in a wide range of crops and plants in natural environments. In order to develop control strategies against Phytophthoraspecies, it is important to k...

Objective: The aim of this study was to estimate genetic variability and phonetic relationships of 50 isolates of P. sojae from Iran. Methods: During 2005–2007, 142 isolates of Phytophthora sojae were collected from soil samples and diseased soybean plants from Lorestan, Mazandaran, Golestan and Ardabil provinces. Races of the isolates were determined. DNA of P. sojae...

The perception of Phytophthora sojae avirulence (Avr) gene products by corresponding soybean resistance (Rps) gene products causes effector triggered immunity. Past studies have shown that the Avr3a and Avr5 genes of P. sojae are genetically linked, and the Avr3a gene encoding a secreted RXLR effector protein was recently identified. We now provide evidence that Avr3a and Avr5 are allelic. Gene...

Plants have evolved pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) and effector-triggered immunity (ETI) to protect themselves from infection by diverse pathogens. Avirulence (Avr) effectors that trigger plant ETI as a result of recognition by plant resistance (R) gene products have been identified in many plant pathogenic oomycetes and fungi. However, the virulence funct...

Phytophthora sojae, an oomycete pathogen of soybean, causes stem and root rot, resulting in annual economic loss up to $2 billion worldwide. Varieties with P. sojae resistance are environmental friendly to effectively reduce disease damages. In order to improve the resistance of P. sojae and broaden the genetic diversity in Southern soybean cultivars and germplasm in the U.S., we established a ...

The molecular mechanisms and the defense responses associated with partial resistance to Phytophthora sojae in soybean are unknown. In this study, we examined correlations between the expression of defense genes with partial resistance. First, to determine whether constitutive levels of expression of defense-related genes correlated with partial resistance to P. sojae, northern blot analysis of...

While genome sequencing is becoming ever more routine, genome annotation remains a challenging process. Identification of the coding sequences within the genomic milieu presents a tremendous challenge, especially for eukaryotes with their complex gene architectures. Here, we present a method to assist the annotation process through the use of proteomic data and bioinformatics. Mass spectra of d...

Understanding the diversity of a pathogen is important for developing disease management recommendations. In Phytophthora root and stem rot–soybean pathosystem, P. sojae characterized into pathotypes based on ability to cause soybean genotypes that each contain different resistance Rps gene. To understand in an area, isolates may be recovered by baiting soil samples. this research, we compared ...

objective: the aim of this study was to estimate genetic variability and phonetic relationships of 50 isolates of p. sojae from iran. methods: during 2005–2007, 142 isolates of phytophthora sojae were collected from soil samples and diseased soybean plants from lorestan, mazandaran, golestan and ardabil provinces. races of the isolates were determined. dna of p. sojae fifty isolates was amplifi...

Root and stem rot disease of soybean is caused by the oomycete Phytophthora sojae. The avirulence (Avr) genes of P. sojae control race-cultivar compatibility. In this study, we identify the P. sojae Avr3c gene and show that it encodes a predicted RXLR effector protein of 220 amino acids. Sequence and transcriptional data were compared for predicted RXLR effectors occurring in the vicinity of Av...