Transgenic mice (K5-PKC alpha) in which the keratin 5 promoter directs the expression of protein kinase C-alpha (PKC alpha) to epidermal keratinocytes display a 10-fold increase in PKC alpha protein in their epidermis and alterations in phorbol ester-induced cutaneous inflammation [J Cell Science 1999;112:3497-3506]. In the current study, we have used these K5-PKC alpha mice to examine the role...

Although lysophosphatidylcholine (LPC)-mediated cellular responses are attributed to the activation of protein kinase C (PKC), relatively little is known about the upstream signaling mechanisms that regulate the activation of PKC and downstream mitogen-activated protein (MAP) kinase. LPC activated p42 MAP kinase and PKC in mesangial cells. LPC-mediated MAP kinase activation was inhibited (but n...

p70S6 kinase (S6K1) plays a pivotal role in hypertrophic cardiac growth via ribosomal biogenesis. In pressure-overloaded myocardium, we show S6K1 activation accompanied by activation of protein kinase C (PKC), c-Raf, and mitogen-activated protein kinases (MAPKs). To explore the importance of the c-Raf/MAPK kinase (MEK)/MAPK pathway, we stimulated adult feline cardiomyocytes with 12-O-tetradecan...

As the most common clinical stress during mid and late pregnancy, antenatal hypoxia has profound adverse effects on individual’s vascular health later in life, but underlying mechanisms are still not understood. The purpose of this study was to reveal acquired dysfunction offspring imposed by hypoxia. Pregnant rats were housed a normoxic or hypoxic (10.5% oxygen) chamber from gestation day 10 2...

Zonula occludens 2 (ZO-2) protein is a tight-junction phos phorylated protein that belongs to the membrane-associated guanylate kinase ('MAGUK') family. Here we study the interaction between ZO-2 and protein kinase C (PKC). We have constructed two ZO-2 fusion proteins of the middle (3PSG) and C-terminal (AP) regions of the molecule and demonstrate that they are phosphorylated by PKC isoenzymes ...

Protein kinase C (PKC) regulates activation of the Raf-1 signaling cascade by growth factors, but the mechanism by which this occurs has not been elucidated. Here we report that one mechanism involves dissociation of Raf kinase inhibitory protein (RKIP) from Raf-1. Classic and atypical but not novel PKC isoforms phosphorylate RKIP at serine 153 (Ser-153). RKIP Ser-153 phosphorylation by PKC eit...

The biochemical basis for the reduced lymphokine production by neonatal T cells compared with adult T cells remains poorly defined. Previous studies have raised the possibility that neonatal T cells could be deficient in their ability to transmit signals via protein kinase (PK) C. We now report that while PKC-dependent activation of the mitogen-activated protein (MAP) kinases, c-Jun N-terminal ...

Activation of Ca2*and phospholipid-dependent protein kinase C (PKC) is associated with increased proliferation in several cell types. When activated, PKC is tightly bound to the particulate cell fraction. Accord ingly, we examined the subcellular distribution of PKC in superficial (nonproliferative) and proliferativi.-colonie epithelial cells from rat colonie mucosa. PKC was determined in solub...

Activation of Ca2+ and phospholipid-dependent protein kinase C (PKC) is associated with increased proliferation in several cell types. When activated, PKC is tightly bound to the particulate cell fraction. Accordingly, we examined the subcellular distribution of PKC in superficial (nonproliferative) and proliferative colonic epithelial cells from rat colonic mucosa. PKC was determined in solubl...