OBJECTIVES Gentamicin is used for the treatment of Gram-negative bacterial infections. However, gentamicin administration is limited because of nephrotoxicity. The aim of the present study was to evaluate the protective effect of crocin against gentamicin-induced nephrotoxicity in rats. MATERIALS AND METHODS Thirty two male Wistar rats received gentamicin (100 mg/kg, IP), with or without croc...

Fifteen gravid, crossbred gilts were utilized in an experiment to measure the effect of five isonitrogenous dietary treatments (12% crude protein, sorghum-soybean meal; sorghum; sorghum + lysine; sorghum + threonine; sorghum + lysine + threonine) on N balance, colostrum composition and plasma constituents (protein, urea N and IgG). All gilts were fed a standard corn-soybean meal diet from breed...

The objective of these studies was to elucidate the causes underlying the reduction of fertility associated with feeding of excess ruminally degradable protein to cattle. Holstein heifers were fed total mixed rations that either met (Normal) or exceeded (High) ruminally degradable protein requirements. In Exp. 1 heifers (n = 80) were inseminated at estrus after being fed the experimental diets ...

The present study was undertaken to investigate the antihyperammonemic efficacy of Withania somnifera root, which has been used in Indian traditional medicine for many centuries. We studied the effect of oral administration of powdered root of W. somnifera (500 mg/kg; b.wt) on blood ammonia, plasma urea, uric acid, non-protein nitrogen and serum creatinine in control and ammonium chloride (AC) ...

We recently reported that in subjects with untreated type 2 diabetes a 5-wk diet of 30:30:40 carbohydrate/protein/fat ratio resulted in a significant decrease in 24-h integrated glucose, total %glycohemoglobin, and total cholesterol compared with a control diet of 55:15:30 carbohydrate/protein/fat given at the beginning of the 5-wk period. Body weight was stable and insulin was unchanged. We no...

Glycine has been regarded as a poor source of nitrogen for total parenteral nutrition. Two prospective randomised cross over controlled clinical trials were undertaken to compare the efficacy of high and low glycine containing amino acid solutions in parenterally fed malnourished hypoalbuminaemic patients with gastrointestinal disease. In the first study (n = 9), amino acid solutions in which g...

The importance of glucagon on postoperative changes in hepatic amino-nitrogen conversion were investigated in six patients undergoing elective cholecystectomy for uncomplicated gall stones. Patients were given infusions of somatostatin (bolus of 6 micrograms/kg followed by continuous infusion of 6 micrograms/kg/h) from induction of anaesthesia to the end of investigation, the first postoperativ...

Three laboratory-prepared urease reagents were compared with a commercial preparation supplied for routine use on the Beckman Blood Urea Nitrogen Analyzer. There were discrepancies in results for urea nitrogen among the four urease reagents when matching serum and the corresponding oxalate/fluoride treated plasma were compared as measured with the Beckman Analyzer and continuous-flow (AutoAnaly...

4. Plasma concentrations and urea entry rates were significantlj and linearly related. 5. The relationship between excretion rate and plasma urea concentration was best described by a cubic equation. 6. Degradation of urea in sheep was found to be extensive in all the animals studied ; as the protein intake increased, the quantity of urea degraded also increased but the percentage of ureaenteri...

We examined whether changes in the gut microbiota induced by clinically relevant interventions would impact the bioavailability of dietary amino acids in neonates. We tested the hypothesis that modulation of the gut microbiota in neonatal pigs receiving no treatment (control), intravenously administered antibiotics, or probiotics affects whole body nitrogen and amino acid turnover. We quantifie...