A survey of Salmonella infections was carried out over a 1-year period in the rural community covered by the Hospital Reina Sofía (Tudela, Spain). The 255 strains that were collected were studied by serotyping, antimicrobial resistance, and plasmid profile analysis. The predominant serotype was S. enteritidis (85.90%), followed by S. typhimurium (7.06%) and S. virchow (2.36%). Only 7.84% of the...

A set of 103 epidemiologically well-defined Acinetobacter baumannii isolates obtained from nine hospital outbreaks and 21 unrelated strains were characterized by pulsed-field gel electrophoresis (PFGE) of total genomic DNA digested with ApaI. Among outbreak strains, eight different patterns and five possible variants were identified by PFGE. Results were compared with those from traditional typ...

plasmids play an important role in the virulence and antibiotic resistance of salmonella. salmonella carry plasmids having different size and number. plasmid profile analysis of single isolates of salmonella typhimurium and salmonella enteritidis revealed the presence of 4- plasmid profiles. salmonella serovars produce several type iii secretions including sop-e and sop –b. soap e is detected b...

The antibiotics resistance pattern, the plasmid profile, the auxotypes and serotypes of 116 Neisseria gonorrhoeae clinical isolates obtained in one year were examined. The incidence of penicillinase producing (PPNG) strains was 12% (14 strains). The most frequent plasmid pattern was the combination of 4.5, 2.6 and 24.5 MDa plasmids. The conjugative plasmid of 24.5 MDa showed a high prevalence (...

Problems associated with typhoid fever epidemic about its diagnosis in developing countries like Nigeria is a perennial healthcare challenge the sector grapples with. Improper of clinical cases have also led to treatment failure and errors as diseases caused by other microorganisms are treated especially result inadequate reliable diagnostic laboratories. A total 3,000 stool specimens from pati...

Two fi-lactamase-producing strains of Neisseria gonorrhoeae were studied. The substrate profile, molecular weight, and isoelectric point of their ,8-lactamases were similar to those of the TEM-1 enzyme produced by many gram-negative bacilli. The gonococcal f8-lactamase was cell bound during exponential growth and was most likely located in the periplasm. Penicillin hydrolysis was efficient in i...

Five of the seven cases of vancomycin-resistant Staphylococcus aureus (VRSA) infection identified to date have occurred in southeastern Michigan. VRSA isolates from the four most recent cases (all from Michigan) were characterized. The vanA gene was localized to a single plasmid in each VRSA isolate. The pulsed-field gel electrophoresis patterns of chromosomal DNA and the restriction profile of...

High resolution melting profiles of four linearised plasmids (pUC9, pGV403, pHP2, and pBR322) were recorded by means of UV absorbance vs. temperature scanning. The set of transitions obtained for each plasmid are compared to each other and to the transitions obtained for their excised particular antibiotica resistance gene. It can be shown that each gene leaves a characteristic mark on the melt...

Escherichia coli is a bacteria belonging to the family Enterobacteriaceae. harmful gram bacterium living in digestive tract. These also live water and soil, commonly referred as coliform. This research aims analyze plasmid profile of its resistance some antibiotics. To explore profiles recent electrophoresis gel, antibiotic used tests Amoxicillin, Tetracycline antibiotic, Ciprofloxacin, Sulfame...

mRNA differential display RT-PCR has been extensively used for the isolation of genes differentially expressed between RNA populations. We have assessed its utility for the identification of developmentally regulated genes in plasmid cDNA libraries derived from individual tissues dissected from early mouse embryos. Using plasmid Southern blot hybridisation as a secondary screen, we are able to ...