plackett-burman design was used for selection of important media components such as carbon and nitrogen sources and minerals which affect poly(hydroxybutyrate) production and cell growth of methylobacterium extorquens dsmz 1340. among the studied variables, nitrogen and phosphorus sources, mgso4 and most of the trace elements were found to be significant variables for phb production from methan...

Cyanobacteria have many unexploited potential for natural products with a huge variability in structure and biological activity. Under stress conditions they are reported to produce biopolymers like poly-β-hydroxybutyrate (PHB), which can be produced intracellularly.Cyanobacteria are capable of synthesizing small amount of poly-β-hydroxybutyrate (PHB) under nitrogen and phosphorous starvation c...

A recombinant Escherichia coli strain has been developed that produces poly(3-hydroxybutyrate-co-4-hydroxybutyrate) when grown in complex medium containing glucose. This has been accomplished by introducing into E. coli DH5 alpha separate plasmids harboring the polyhydroxyalkanoate (PHA) biosynthesis genes from Ralstonia eutropha (formerly named Alcaligenes eutrophus) and the succinate degradat...

mathematical modeling and simulation of microbial polyhydroxybutyrate (phb) production process is beneficial for optimization, design, and control purposes. in this study a batch model developed by mulchandani et al., [1] was used to simulate the process in matlab environment. it was revealed that the kinetic model parameters were estimated off the optimal or at a local optimal point. therefore...

We have identified two Sinorhizobium meliloti chromosomal loci affecting the poly-3-hydroxybutyrate degradation pathway. One locus was identified as the gene acsA, encoding acetoacetyl coenzyme A (acetoacetyl-CoA) synthetase. Analysis of the acsA nucleotide sequence revealed that this gene encodes a putative protein with a molecular weight of 72,000 that shows similarity to acetyl-CoA synthetas...