Polydimethyl siloxane (PDMS)–glass microchip has a very strong surface effect on polymerase chain reaction (PCR), leading to a very poor PCR yield. In the work reported here, practical dynamic passivation of surfaces of PDMS–glass microchip using polyethylene glycol (PEG) or polyvinylpyrrolidone (PVP) was achieved using a conventional thermocycler. The passivation procedure was cost-effective a...

The completion of human genome sequencing has shifted the focus of research from genes to proteins. In this regard, a protein library chip has become a useful tool for cell-free protein synthesis. In this study, we attempted to make a highly-integrated protein chip from a DNA library using in vitro protein synthesis on a microchamber array fabricated by using PDMS (polydimethyl siloxane), a hyd...

An integrated tunable microlens, whose focal length may be varied over a range of 3 to 15 mm with total power consumption below 250 mW, is presented. Using thermo-pneumatic actuation, this adaptive optical microsystem is completely integrated and requires no external pressure controllers for operation. The lens system consists of a liquid-filled cavity bounded by a distensible polydimethyl-silo...

Astrogliosis in a dish: Substrate stiffness induces astrogliosis in primary rat astrocytes" (2016). Astrogliosis due to brain injury or disease can lead to varying molecular and morphological changes in astrocytes. Magnetic resonance elastography and ultrasound have demonstrated that brain stiffness varies with age and disease state. However, there is a lack in understanding the role of varied ...

Fluid-supported lipid bilayers self-assemble on glass and SiO2 surfaces. We have found that it is also possible to assemble fluid bilayers on plasma-oxidized polydimethyl siloxane (PDMS) surfaces. Furthermore, it is possible to transfer or print the supported bilayer from raised PDMS surfaces, such as are typically used for microcontact printing, to fresh glass surfaces creating a supported bil...

A flexible amperometric glucose sensor formed on biocompatible polymer was fabricated and tested. The sensor was fabricated by laminating biocompatible polymers and film electrodes. Functional and biocompatible polymers of polydimethyl siloxane (PDMS) and 2-methacryloyloxyethyl phosphorylcholine (MPC) copolymerized with dodecyl methacrylate (DMA) were utilized as the sensor material. Particular...

The volatile components of Citrus aurantium L. flowers were characterized by GC-MS with two different extraction techniques, hydrodistillation (HD) and ultrasonic-assisted headspace solid phase microextraction (UA-HS-SPME). In the SPME method, the volatile components of the samples, irradiated by ultrasonic radiation, were collected on a polydimethyl siloxane (PDMS) commercial fiber as...

We report a laminar flow-based microfluidic bioelectrochemical system (BES) that was fabricated by using polydimethyl siloxane (PDMS) channels and gold electrodes. The microfluidic BES was operated as a potentiostatically controlled two-electrode system. A pure culture of Geobacter sulfurreducens strain PCA, which is a model electrode-respiring bacterium, was grown in the channel and respired w...