the genetic diversity of 40 tobacco (nicotiana tabacum l.) cultivars was analyzed using 12 issr primers. thecultivars were evaluated for 14 traits using a simple lattice design (7×7) with two replications. of 149 amplified bands, 142 (95.3%) were polymorphic. maximum and minimum bands were generated by primers ubc825 (17 bands and 94.11% polymorphic) and ubc824 (7 bands and 100% polymorphic), r...

conclusions this is the first research in comparing two genetic marker systems in p. variotti. we were prompted to explore polymorphisms utility in p. variotti with a look at using germplasm screening mapping of genome and strain improvement programs. results both systems discriminated 20 isolates of p. variotii successfully but were different in the amount of detectable polymorphism. using clu...

Allelic variation at 46 simple sequence repeat (SSR) marker loci well distributed across the sorghum genome was used to assess genetic diversity among 92 sorghum lines, 74 resistant and 18 susceptible to grain mould. Of the 46 SSR markers, 44 were polymorphic, with the number of alleles ranging from 2 to 20 with an average of 7.55 alleles per locus. Genetic diversity among the sorghum lines was...

Whole genome sequence in mango offers unprecedented opportunities for genomics assisted crop improvement via enabling access to genome-wide genetic markers. In the present study, simple repeats (SSRs) were predicted from of mango. Based on SSR length (? 50 bp), highly-variable SSRs (MSSRs) sorted. A sub-set 129 MSSRs was validated a set 24 diverse genotypes yielding marker validation and polymo...

The objective of this work was to standardize a semiautomated method for genotyping soybean, based on universal tail sequence primers (UTSP), and to compare it with the conventional genotyping method that uses electrophoresis in polyacrylamide gels. Thirty soybean cultivars were genotypically characterized by both methods, using 13 microsatellite loci. For the UTSP method, the number of alleles...

the present study was to construct a parentage verification system for iranian caspian horse. a total number of 45 caspian horse samples including 14 foals for parentage verification, 17 stallion and 14 mare for individual identification were genotyped. genomic dna was extracted from whole blood and the genotype were analysed by pcr procedure and using 7 microsatellite markers (aht04, hms03, hm...

In this study, Simple Sequence Repeat markers (SSR) were used to investigate the genetic variation between 49 cumin ecotypes collected from 9 different provinces of Iran. SSR primers Elap1479, Elap040 and Elap1493 showed the highest (89%), while Elap1340 and Elap017 showed the lowest (56%) number of polymorphic bands. Polymorphism information content (PIC) values varied between 0.18 - 0.37. The...

Montipora digitata is a species belonging to the Acroporidae. In Indo-Pacific region, M. widely distributed and dominant of scleractinian coral in South China Sea, however, there are currently no molecular markers suitable for assessing genetic diversity. Here, restriction-site associated DNA sequencing (RAD-seq) was used isolate characterize polymorphic microsatellite loci. A total 317,361 RAD...