Analysis of genetic diversity among genotypes and differentiation populations are crucial for determination conservation strategies one the best plant breeding approaches. In this study, ISSR markers were used to determine variation population structure in 23 hulled wheats. Together with control durum wheat bread registered cultivars, 32 analyzed 14 located throughout genome. Number alleles per...

Cowpea improvement has continued to play key roles in the quest for national food security. This work elucidated molecular genetic diversity and phylogenetics among four released cowpea varieties (FUAMPEA-1, FUAMPEA-2, FUAMPEA-3 AND FUAMPEA-4). DNA was extracted from 14 day old seedlings using CTAB method. A total of nine (9) SSR (Simple Sequence Repeats) markers were employed amplifications. B...

in the present study, genotyping of six short tandem repeat (str) loci including csf1po, d16s539, f13a01, f13b, lpl and hprtb was performed on genomic dna from 127 unrelated individuals from the iranian province of isfahan. the results indicated that the allele and genotype distributions were in accordance with hardy-weinberg expectations. the observed heterozygosity (ho), expected heterozygosi...

Two commercial broiler lines were used in an experiment to map quantitative trait loci (QTL) affecting disease resistance. Chickens from these lines were genotyped with 27 microsatellite markers to estimate heterozygosity and polymorphism information content (PIC), the probability that one parent is heterozygous at a marker locus and the other has a different genotype. Heterozygosity estimated ...

Spinach (Spinacia oleracea L.) is an economically important leafy vegetable crop in many countries. This is the first case study of using SRAP markers to analyze genetic diversity of Iranian spinach accessions. Eight SRAP primer combinations generated 88 scorable bands ranging from 50 to 1000 bp, among which 73 were polymorphic, with an average of 82.9 polymorphic bands per primer combination a...

In the present study, genotyping of six short tandem repeat (STR) loci including CSF1PO, D16S539, F13A01, F13B, LPL and HPRTB was performed on genomic DNA from 127 unrelated individuals from the Iranian province of Isfahan. The results indicated that the allele and genotype distributions were in accordance with Hardy-Weinberg expectations. The observed heterozygosity (Ho), expected heterozygosi...

iron is one of the most important nutrients in the human diet. according to the high consumption of staple foods such as wheat, the deficiency of iron in these crops would lead to nutritional disorders and related complications. to identify microsatellite markers associated with wheat grain iron content,38iranian prevalent wheat genotypes were assessed using 30 pairs of genomic and est microsat...

The present study was hypothesized to evaluate a set of SSRs for the assessment genetic variations in Asparagus officinalis L. and their allied species. Nine genic SSR markers were especially developed genome employed DNA profiling studies These have revealed allelic polymorphism ranging from 1.0 2.0. Allele frequency found highest psbD-trnL (1.0), petB (1.0) AG7 while it lowest ZHD1 (0.1). Pol...

genotyping for five short tandem repeat (str) loci humvwa, humfes, humtpo, humth01 and d3s1359 was done in 220 unrelated individuals from the population of isfahan province of ir iran. the loci were genotyped using the polymerase chain reaction (pcr) followed by polyacrylamide gel electrophoresis (page) and silver staining. the data demonstrated that the str markers were all found informative i...

the genetic diversity of 89 flue-cured tobacco varieties was examined using 12 issr primers. these cultivarswere evaluated at the guilan tobacco research center, rasht, iran, using a 7×7 simple lattice design with two replications, and 12 morphological traits. the total number of pcr amplified products was 143 bands ranging from 450 to 3000 bp, of which 108 bands (74.28%) were polymorphic. prim...