Vi polysaccharide from Salmonella enterica serotype Typhi is used as one of the available vaccines to prevent typhoid fever. Measurement of Vi-specific serum antibodies after vaccination with Vi polysaccharide by enzyme-linked immunosorbent assay (ELISA) may be complicated due to poor binding of the Vi polysaccharide to ELISA plates resulting in poor reproducibility of measured antibody respons...

Methods were developed for the polyacrylamide gel electrophoretic analysis of capsular polysaccharides of bacteria with Escherichia coli K1 as a model. Conditions were determined for the rapid and gentle extraction of the K1 polysaccharide by incubation of the bacteria in a volatile buffer and for the subsequent removal of the putative phospholipid moiety attached to the reducing end of the pol...

Terminalia chebula, a medicinal plant, is widely used in the management of various diseases. As the water extract of its dried ripe fruit is a frequently used preparation, we decided to look for bioactive polysaccharide in this extract. We demonstrate that the obtained polysaccharide fraction, CP, contained a highly branched arabinogalactan protein having a (1 → 3)-, (1 → 6)- and (1 → 3, 6)-lin...

The type III polysaccharide of -roup B Streptococcus has been isolated and purified by a method that employs washing of intact cells at neutral pH. That the polysaccharide prepared by this procedure is the "native" type III antigen is suggested by its molecular size in excess of 10(6) daltons, its degradation by acid and heat treatment to a fragment with immunologic characteristics of the class...

The occurrence in the rabbit of an acute phase protein analogous to human C-reactive protein has been confirmed. The acute phase protein of the rabbit reacts with a special form of the pneumococcal somatic polysaccharide, designated Cx polysaccharide, in the same manner that the human C-reactive protein reacts with the classical C polysaccharide. The method of preparation and some of the proper...

The interaction of proteins with polysaccharides represents a major and challenging topic in glycobiology, since such complexes mediate fundamental biological mechanisms. An affinity capillary electrophoresis method has been developed to evidence the complex formation and to determine the binding properties between an anticoagulant polysaccharide of marine origin, fucoidan, and a potential targ...

the alginate was extracted with six different methods from iran south seacoast algae, sargassum sp, and the percentage yield of alginate was determined. we divided our methods to two groups including acidic extraction and non-acidic. in acidic methods, hcl and h 2 so 4 were used as a detergent in extraction process and cacl 2 was exerted in non-acidic treatments. all treatments compared with ea...

Erythrocytes treated with polysaccharide from Past. tularensis are specifically agglutinated by the sera of persons recovered from tularemia or vaccinated against it, and by the sera of animals immunized with living cultures. The serum hemagglutination titer is correlated with the amount of antibody as measured by the quantitative precipitin method with polysaccharide, but not with the bacteria...

BACKGROUND Group A Neisseria meningitidis has been a major cause of bacterial meningitis in the sub-Saharan region of Africa in the meningitis belt. Neisseria meningitidis is an encapsulated pathogen, and antibodies against the capsular polysaccharide are protective. Polysaccharide-protein conjugate vaccines have proven to be highly effective against several different encapsulated bacterial pat...