avian pasteurella multocida is an agent of fowl cholera. the protective effect achieved through orthodoxvaccines is not ideal. the research on novel vaccines against avian pasteurella multocida is imperative. inthis study, the genes encoding outer membrane protein h and a (omph and ompa) were cloned into theeukaryotic expression vector pcdna3.1(+) and the recombinant plasmids, namely dna vaccin...

conclusions the pc-ns3 possesses the capacity to express ns3 in the mammalian cell line and demonstrated strong immunogenicity in a murine model. our primary results demonstrated that the immunogenic truncated region of ns3 could be used as a potential vaccine candidate against hepatitis c. results the pcdna3.1 plasmid harboring the coding sequence of ns3 (pc-ns3) was constructed and confirmed ...

background: cervical cancer is the most prevalent tumor in developing countries and the second most frequent cancer among female population worldwide. specific human papillomaviruses and, most notably, hpv types 16 and 18 are recognized as being caus-ally associated with cervical carcinomas. the early hpv type 16 genes, e6 and e7, di-rectly participate in the in vitro transformation of primary ...

conclusions the results revealed that the novel multi-epitope dna construct could be an effective candidate in tuberculosis vaccine development, and it is qualified to investigate its potential to induce cd4 and cd8 t- cell immune response in the experimental animal model. results mhc class i-and class ii-predicted epitopes showed a high potential to binding to human hlas alleles, with global b...

Background: Mycobacterium tuberculosis is the causative agent of tuberculosis (TB). Bacille Calmette-Guerin (BCG) vaccine, is not effective in adults, therefore, many efforts have been made to produce an effective adult TB vaccine. The aim of this study was to develop a new tuberculosis DNA vaccine candidate encoding a recombinant HspX-PPE44-EsxV fusion antigen of M. tuberculosis. Methods: ...

background hypervariability of hcv proteins is an important obstacle to design an efficient vaccine for hcv infection. multi-epitope vaccines containing conserved epitopes of the virus could be a promising approach for protection against hcv. objectives cellular and humoral immune responses against multi-epitope dna and peptide vaccines were evaluated in balb/c mice. materials and methods in th...

Objective(s): Tuberculosis (TB) has still remained a global health issue. One third of the world's population is infected with tuberculosis and the current BCG vaccine has low efficiency; hence, it is necessary to develop a new vaccine against TB. The aim of the current study was to evaluate the efficiency of a novel DNA vaccine encoding Mtb32C-HBHA antigen in inducing specific immune responses...

Objective(s): Infection with tuberculosis (TB) is regarded as a major health issue. Due to the emergence of antibiotic resistance during TB treatment, prevention via vaccination is one of the most effective ways of controlling the infection. DNA vaccines are developed at a greater pace due to their ability in generating a long-lasting immune response, higher safety com...

We introduce the interval order polytope of a digraph D as the convex hull of interval order inducing arc subsets of D. Two general schemes for producing valid inequalities are presented. These schemes have been used implicitly for several polytopes and they are applied here to the interval order polytope. It is shown that almost all known classes of valid inequalities of the linear ordering po...