Potyviruses are major pathogens that often cause mixed infection in calla lilies. To reduce the time and cost of virus indexing, a detection method for the simultaneous targeting of multiple potyviruses was developed by generating a broad-spectrum monoclonal antibody (MAb) for detecting the greatest possible number of potyviruses. The conserved 121-amino-acid core regions of the capsid proteins...

Mixed viral infections in plants involving a potyvirus and other unrelated virus often result synergistic effects, with significant increases accumulation of the non-potyvirus partner, as case melon infected by Watermelon mosaic (WMV) crinivirus Cucurbit yellow stunting disorder (CYSDV). To further explore interaction between these two viruses, activity RNA silencing suppressors (RSSs) was addr...

Potyviruses (genus Potyvirus, family Potyviridae) are transmitted by aphids in a non-persistent manner and cause significant losses in many crops including bulbous ornamentals. Host range, symptoms, physical and biochemical properties of many potyviruses in bulbous ornamentals are reported, but, especially for viruses infecting ornamentals of minor economical importance, sequence data are still...

Abstract Passionfruit ( Passiflora edulis ) is widely cultivated in tropical and subtropical regions around the world. Several viruses of genus Potyvirus pose serious threat to passion fruit production. The origin, dispersal evolution these potyviruses, however, are poorly understood. Here, we investigated genetic structure telosma mosaic virus (TelMV), a potyvirus that infects passionfruit Eas...

A small open reading frame (ORF), pipo, overlaps with the P3 coding region of the potyviral polyprotein ORF. Previous evidence suggested a requirement for pipo for efficient viral cell-to-cell movement. Here, we provide immunoblotting evidence that the protein PIPO is expressed as a trans-frame protein consisting of the amino-terminal half of P3 fused to PIPO (P3N-PIPO). P3N-PIPO of Turnip mosa...

Coat protein (CP) gene of sugarcane streak mosaic virus-AP isolate (SCSMV-AP) was expressed in E. coli and recombinant CP (SCSMV-AP rCP) was purified by linear sucrose density gradient centrifugation. Observation of purified SCSMV-AP rCP under electron microscope revealed the presence of potyvirus-like particles (PVLPs). The assembled particles were shown to encapsidate CP gene transcripts by s...

The potyvirus group [named after its type member, potato virus Y (PVY)] is the largest of the 34 plant virus groups and families currently recognized (Ward & Shukla, 1991). It contains at least 180 definitive and possible members (or 30% of all known plant viruses) which cause significant losses in agricultural, pasture, horticultural and ornamental crops (Ward & Shukla, 1991). These viruses ar...