the way to overcome current limitations in the generation of glucose-responsive insulin-producing cells is selective enrichment of the number of definitive endoderm (de) progenitor cells. sox17 is the marker of mesendoderm and definitive endoderm. the aim of the present research was to study the potential of sox17 knock-in cgr8 mouse embryonic stem (es) cells to differentiate into insulin produ...

objective: crispr/cas9 technology provides a powerful tool for targeted modification of genomes. in this system, a donor dna harboring two flanking homology arms is mostly used for targeted insertion of long exogenous dna. here, we introduced an alternative design for the donor dna by incorporation of a single short homology arm into a circular plasmid. materials and methods: in this experiment...

behaviour studies indicated that the permethrin resistant strin of an. stephensi was 3-fold resistant to knock-down compared with the susceptible strain. the resistant strain was however 3-fold less irritable to permethrin and less responsive than the susceptible strain to the movement of an aspirator. if reduced irritability and reduced responsiveness to catch are consequences of the changes i...

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Objectives:The expression levels of heat shock proteins (HSPs) are elevated in many cancers, and this overexpression is often associated with both a poor survival and a therapeutic outcome. Curcumin is an anti-cancer agent that also induces a heat shock response. HSPs confer resistance to curcumin-induced apoptosis in cancerous cells. The aim of the current study was to analyze variations in th...

objective(s):eukaryotic translation initiation factor 4e (eif4e) is overexpressed in cervical cancer (cc). however, the molecular mechanisms are unclear. this study aimed to investigate the molecular mechanism of eif4e gene overexpression in cc. materials andmethods:the human papillomavirus (hpv) type 18 e7 and eif4e mrnas were measured following knock down or overexpression of e7 gene by rt-pc...

the pediatric risk of mortality (prism) score is a measure of illness severity based on abnormalities observed on bedside examination and laboratory assessment at pediatric intensive care unit (picu) admission to predict mortality probability. our study was performed on 205 patients who were admitted to picu of children’s medical center (cmc) over a period of 6 months.data were recorded prospec...