the objective of this study was to evaluate the effect of the sto feeder layer on prepubertal rhode island red rooster sscs culture and proliferation in vitro. testis cells from 30 prepubertal rhode island red chicken (4-8 weeks of age), were individually separated and cultivated in the presence of  bfgf and lif growth factors on four well plates with two treatments and three replicats and five...

In this report we document the creation of transgenic mice in which the native ratio of A and B forms of progesterone receptor (PR) has been altered by the introduction of additional A form as transgene. We also show that in these mice there is an aberration in mammary development. In ovariectomized prepubertal PR-A transgenic mice, end buds with unusual morphology persist after ovariectomy, an...

The aim of this study is to describe the clinical characteristics of prepubertal- and adolescent-onset bipolar disorder (BD) and to identify any clinical differences between patients with prepubertal- and adolescent-onset BD. We analyzed the clinical records of 53 inpatients with BD. These patients were divided into prepubertal-onset and adolescent-onset groups. We also divided the subjects int...

The GnRH neurons represent the output cells of the neuronal network controlling gonadal function. Their activation initiates the onset of puberty, but the underlying mechanisms remain unclear. Using a GnRH-green fluorescent protein mouse model, we have been able to fill individual GnRH neurons with biocytin in the acute brain slice preparation to examine their morphological characteristics acro...

The association between radiographic evidence of mandibular asymmetry and history of prepubertal trauma was analyzed in orthognathic surgery patients and orthodontic patients. There were statistically significant associations between radiographic evidence of mandibular asymmetry and a history of prepubertal trauma in both patient groups. The results suggest that prepubertal trauma could be one ...

The classic view of brain and behavioral sexual differentiation holds that the neural mechanisms controlling sexual behavior in female rodents develop in the absence of ovarian sex hormone actions. However, in a previous study, female aromatase knock-out (ArKO) mice, which cannot convert testosterone to estradiol, showed deficient male-oriented partner preference and lordosis behaviors in respo...

Testosterone, after being converted to estradiol in the brain, acts on estrogen receptors (ERα and ERβ) and controls the expression of male-type social behavior. Previous studies in male mice have revealed that ERα expressed in the medial preoptic area (MPOA) and medial amygdala (MeA) are differently involved in the regulation of sexual and aggressive behaviors by testosterone action at the tim...

BACKGROUND common use of sevoflurane in congenital defects during repeated surgeries may have detrimental effects on spermatogenesis after puberty. OBJECTIVE This study investigated sevoflurane effects on spermatogenesis process in male mature mice after exposure in prepubertal time. MATERIALS AND METHODS 24 neonatal NMRI male mice were randomly classified in three groups. Experimental 1 an...

Our aim is to verify if oocyte developmental potential is related to the timing of meiotic progression and to mitochondrial distribution and activity using prepubertal and adult oocytes as models of low and high developmental capacity respectively. Prepubertal and adult oocytes were incorporated in an in vitro maturation system to determine meiotic and developmental competence and to assess at ...