We have explored the microbial community in a nonpermafrost, cold Alaskan soil using both culture-based and culture-independent approaches. In the present study, we cultured >1000 bacterial isolates from this soil and characterized the collection of isolates phylogenetically and functionally. A screen for antibiosis identified an atypical, red-pigmented strain of Janthinobacterium lividum (stra...

UNLABELLED Serratia marcescens generates secondary metabolites and secreted enzymes, and it causes hospital infections and community-acquired ocular infections. Previous studies identified cyclic AMP (cAMP) receptor protein (CRP) as an indirect inhibitor of antimicrobial secondary metabolites. Here, we identified a putative two-component regulator that suppressed crp mutant phenotypes. Evidence...

Prodigiosin, a secondary metabolite extracted from Serratia marcescens (S. marcescens), could induce apoptosis in various cancer cells, with however low toxicity on normal cells. The red pigment was strain S. NJZT-1 isolated soil, which had antibacterial activity. Spectral analyses (LC-ESI-MS, UV-VIS spectrophotometry, infrared spectra and HPLC) TLC indicated the presence of prodigiosin extrace...

Natural products either synthesized or secreted by organisms represent one of the critical sources of potential medicinal use. Both natural pigments and synthetic dyes have been widely used in different fields in on a daily basis life such as foods or feeds, textiles, cosmetics, pharmaceuticals, paper printing inks, etc. Secondary metabolites of bacterial origin include various enzymes, pigment...

Prodigiosins, a family of natural red pigments characterized by a common pyrrolylpyrromethane skeleton, are produced by various bacteria that first characterized from Serratia marcescens. This pigment is a promising drug owing to its reported characteristics of having antifungal, immunosuppressive and anti-proliferative activity. From an industrial point of view to obtain optimal conditions to ...

Synthetic dyes are hazardous to the environment and humans due their toxic recalcitrant nature. Hence, present study attempts produce eco-friendly dye from sago industrial waste using S. marcescens SS1. The produced by bacterial bioconversion of was characterized GC-MS obtained 13.423 acquisition time, which is similar standard prodigiosin. effects dyeing parameters were optimized for Jute fabr...

The natural product prodigiosin 1, often described as an H+/Cl- symport cotransporter, can transport Cl- across lipid vesicles via an anion exchange (or antiport) mechanism.

We reported previously [Kataoka, Muroi, Ohkuma, Waritani, Magae, Takatsuki, Kondo, Yamasaki and Nagai (1995) FEBS Lett. 359, 53-59] that prodigiosin 25-C (one of the red pigments of the prodigiosin group produced by micro-organisms like Streptomyces and Serratia) uncoupled vacuolar H+-ATPase, inhibited vacuolar acidification and affected glycoprotein processing. In the present study we show tha...

Prodigiosin is the only pigment of Serratia that has been subjected to chemical analysis.2' 3 The absorption spectrum of prodigiosin as isolated by Wrede has been presented by Ehrismann and Noethling.4 It has been claimed" that the chemically prepared pigment from the ether extract as obtained by Wrede can be further resolved into two components by column chromatography. The absorption spectrum...

We reported previously (Kataoka, T., Muroi, M., Ohkuma, S., Waritani, T., Magae, J., Takatsuki, A., Kondo, S., Yamasaki, M., and Nagai, K. (1995) FEBS Lett. 359, 53-59) that prodigiosin 25-C uncoupled vacuolar H+-ATPase, inhibited vacuolar acidification, and affected glycoprotein processing. In the present study we show that prodigiosins (prodigiosin, metacycloprodigiosin, and prodigiosin 25-C)...