Differential protein precipitation is a rapid and economical step in protein purification and is based on exploiting the inherent physicochemical properties of the polypeptide. Precipitation of recombinant proteins, lysed from the host cell, is commonly used to concentrate the protein of choice before further polishing steps with more selective purification columns (e.g., His-Tag, Size Exclusio...

Central Europe was characterized by a humid-temperate climate in the 20(th) century. Climate change projections suggest that climate in this area will shift towards warmer temperatures by the end of the 21(st) century, while projected precipitation changes are highly uncertain. Here we show that the 2015 summer rainfall was the lowest on record since 1901 in Central Europe, and that climate mod...

The main source of error in the determination of bilirubin in serum by the diazo reaction has been the loss caused by adsorption on the protein precipitate. In addition, the lack of an accurately matching artificial standard and the sensitivity of the azobilirubin color to changes in pH have made accurate calorimetric determinations impossible, without the aid of some type of objective photomet...

At an intraspecific level, patterns of species abundance distribution (SAD) vary in space and time, especially over the largest scales. The most basic, unimodal pattern is expressed in a log-normal shape, based on the abundant-center hypothesis (Gaston et al. 2008). SAD patterns can be driven by environmental variables (e.g. temperature, salinity, precipitation, productivity) as well as intrins...

Understanding recent biogeographic responses to climate change is fundamental for improving our predictions of likely future responses and guiding conservation planning at both local and global scales. Studies of observed biogeographic responses to 20th century climate change have principally examined effects related to ubiquitous increases in temperature - collectively termed a warming fingerp...

The agro-climatic environments in subtropical islands around Japan were analyzed by using meteorological data (average for 1971–2000). This study found that the horizontal distribution of monthly mean temperatures in both the subtropical islands and Japan’s main islands can be expressed accurately as a function of latitude for all seasons. These relationships cannot be applied to the monthly me...

Species range boundaries often form along environmental gradients that dictate the success of the phenotypes present in each habitat. Sociality may allow colonization of environments where related species with a solitary lifestyle cannot persist. Social spiders in the genus Anelosimus appear restricted to low- and mid-elevation moist environments in the tropics, while subsocial spiders, common ...

This study is devoted to the evaluation of the role of assimilation of conventional data on the quantitative precipitation forecasts at regional scale. The conventional data included surface station reports as well as upper air observations. The analysis was based on the simulation of 15 cases of heavy precipitation that occurred in the Eastern Mediterranean. The verification procedure revealed...

Monthly mean air temperature and precipitation data, monthly dust storm frequencies (times of dust storm occurrences) of 107 stations in North China were analyzed to clarify the relationship between the climate change and dust storm occurrence. Arid regions of North China were divided into five and trends of dust storm occurrence and climate changes showed different patterns for these regions, ...

Research that connects the effects of urbanization on biodiversity and ecosystem services is lacking. Ants perform multifarious ecological functions that stabilize ecosystems and contribute to a number of ecosystem services. We studied responses of ant communities to urbanization in the Lake Tahoe basin by sampling sites along a gradient of urban land development. We sampled ant communities, me...