introduction: manipulation of protein stability is important for understanding the principles that govern protein thermostability, both in basic research and industrial applications. various data mining techniques exist for prediction of thermostable proteins. furthermore, ann methods have attracted significant attention for prediction of thermostability, because they constitute an appropriate ...

administering growth hormone increases growth rate of cultured fish. the aim of this study was isolation and synthesize of persian sturgeon gh cdna. the total rna was extracted from pituitary gland of persian sturgeon and cdna was synthesized. the full-length cdna sequence of persian sturgeon contains a 645 nucleotide open reading frame, which encoding a 214 amino acid residues. the position of...

background: sigma factors are proteins that regulate transcription in bacteria. sigma factors can be activated in response to different environmental conditions. the rpos (rna polymerase, sigma s) gene encodes sigma-38 (σ38, or rpos), a 37.8 kda protein in pseudomonas aeruginosa (p. aeruginosa) strains. rpos is a central regulator of the general stress response and operates in both retroactive ...

PrPC conversion to PrPSc isoform is the main known cause for prion diseases including Crutzfeldt-Jakob, Gerstmann-Sträussler-Sheinker syndrome and fatal familial insomnia in human. The precise mechanism underling this conversion is yet to be well understood. In the present work,  using the coordinate file of PrPC (available on the Protein Data Bank) as a starting structure, separate molecular d...