The amino acid sequence of the M-protein for multiple myeloma is unique compared to polyclonal antibodies in patients’ blood. This uniqueness exploited develop an ultrasensitive detection method utilizing mass spectrometry (MS). involves de novo sequencing full-length M-protein, and a targeted MS/MS assay detect quantify serum samples. Healthy control spiked with NISTmAb serial samples from MM ...

Objective(s): Helicobacter pylori, a human specific gastric pathogen is a causative agent of chronic active gastritis. The vacuolating cytotoxin (VacA) is an effective virulence factor involved in gastric injury. The aim of this study was to construct a recombinant protein containing antigenic region of VacA gene and determine its antigenicity.   Materials and Methods: The antigenic region of V...

abstract background and aims: influenza a(h5n1) viruses  circulating in animals might evolve and acquire the ability to spread from  human to human and thus start a pandemic. hemagglutinin (ha) has been shown to play a major role in binding of influenza virus to its target cell and the main neutralizing antibody responses elicit against this region. recent studies have shown that glycosylation ...

background and aims: influenza a(h5n1) viruses  circulating in animals might evolve and acquire the ability to spread from  human to human and thus start a pandemic. hemagglutinin (ha) has been shown to play a major role in binding of influenza virus to its target cell and the main neutralizing antibody responses elicit against this region. recent studies have shown that glycosylation of ha is ...

conclusions the pe gene was successfully cloned and confirmed by sequencing. finally, pe was obtained with high concentration. due to high homology and similarity among the pe gene from nthi atcc 49766 and other nthi strains in genbank, we believe that the protein is a universal antigen to be used as a vaccine design candidate and further studies to evaluate its immunogenicity is underway. mate...

Background: Ataxia with oculomotor apraxia type 1 (AOA1) shows early onset with autosomal recessive inheritance and is caused by a mutation in the aprataxin (APTX) gene encoding for the APTX protein. Methods: In this study, a 7-year-old girl born of a first-cousin consanguineous marriage was described with early-onset progressive ataxia and AOA, with increased cholesterol concentration and decr...

Infectious bursal disease virus (IBDV) is the causative agent of Gumboro disease, an infectious disease of global economic importance in poultry. Structural protein VP2 of IBDV is the most frequently studied protein due to its significant roles in virus attachment, protective immunity, and serotype specificity. The objective of the present study was to improve the expression of hypervariable re...

The Hepatitis B Surface antigen ( HBsAg) gene was isolated from an Iranian HBeAg positive carrier by PCR. The gene was cloned in pUC19 for sequencing and pYES2 for expression in Saccharomyces cerevisiae, which pNF1 and pDF3 constructs were made respectively. The sequencing data showed that the isolated HBsAg gene shared more than 90% homology with the ayw subtype. The pDF3 was transferred into ...