the hemopoietic activity of the human placenta was studied in vitrc under various conditions in order to assess the optimal conditions of protein synthesis and colony stimulating factor (csf) production by this tissue. the hemopoietic activity of placental tissue was assayed by the semi -solid agar medium technique. in order to obtain maximum hemopoietic activity, various stimulators were exami...

the impact of pgf2? on total proteins, free amino acids and the amino transferases in the gonads of silkworm, bombyx mori l. were investigated in the present experiment. the effect of pgf2? resulted in increase in the total protein and free amino acid contents in the gonads of silkworm larvae indicating improved structural and dynamic levels of organization. the enhanced activity levels of amin...

This study evaluates whether protein synthesis plays a role in targeting RNA molecules to different subcellular domains within neurons. Transport of newly synthesized RNA (labeled with [3H]uridine) was examined in the presence of the protein synthesis inhibitors puromycin and cycloheximide. In situ hybridization was used to determine whether inhibition of protein synthesis altered the subcellul...

recombinant protein purification is a kind of sensitive and expensive method in genetics engineering. genetic manipulation leads to the expression of various proteins; it should be isolated with high purity finally. differed methods for protein purification are categorized, based on cast, quality, easy work and side effect of protein. in this article, we are investigating his his-tag protein pu...

Synchronization of rumen available protein and energy is one of the conceptual methods to increase the efficiency of utilization of nutrients by the ruminants The concept of synchronization energy and protein was first by Jhonson, Implying that maximum microbial protein synthesis could be achieved by matching the rate of Organic Matter and protein degradation. Synchronization of rumen available...

The administration of 1,25-dihydroxyvitamin D, (1,25(OH),D,) to rachitic chicks produces an increase in (a) RNA and protein synthesis, (b) calcium binding protein (CaBP) concentration, and (c) alkaline phosphatase activity in the duodenum. These events occur concomitantly with enhanced calcium transport. We inhibited RNA and protein synthesis in rachitic chicks and measured the subsequent respo...

The administration of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) to rachitic chicks produces an increase in (a) RNA and protein synthesis, (b) calcium binding protein (CaBP) concentration, and (c) alkaline phosphatase activity in the duodenum. These events occur concomitantly with enhanced calcium transport. We inhibited RNA and protein synthesis in richitic chicks and measured the subsequent respo...

The rates of protein synthesis and degradation and of amino acid transport were determined in the leg muscle of untrained postabsorptive normal volunteers at rest and approximately 3 h after a resistance exercise routine. The methodology involved use of stable isotopic tracers of amino acids, arteriovenous catheterization of the femoral vessels, and biopsy of the vastus lateralis muscle. During...