proteomics concerns itself with the characterization and function of all cellular proteins, the ultimate determinants of cellular function. mass spectrometry has emerged as the preferred method for in-depth characterization of the protein components of biological systems. using mass spectrometry, key insights into the composition, regulation and function of molecular complexes and pathways have...

Proteomics encompasses several disciplines in the quest to unravel the science of life. Mass spectrometry plays a key rôle in proteomics-associated analyses and this review describes that function in terms of the instrumentation used, the results generated, data interpretation and the advances in analytical techniques being made to deal with the production of highly specific, high throughput da...

Enabling technologies for proteomics (studies examining the protein complement of the genome) have been in development for over 20 years. However, more recently, the field has become formalized by combining the techniques for large-scale protein separation (two-dimensional electrophoresis) with very precise, high fidelity approaches to the analysis and characterisation of the separated proteins...

Systems biology approaches including proteomics are becoming more widely used in cardiovascular research. In this review article, we focus on the application of proteomics to the cardiac extracellular matrix (ECM). ECM remodelling is a hallmark of many cardiovascular diseases. Proteomic techniques using mass spectrometry (MS) provide a platform for the comprehensive analysis of ECM proteins wit...

Plant pathogenic fungi cause important yield losses in crops. In order to develop efficient and environmental friendly crop protection strategies, molecular studies of the fungal biological cycle, virulence factors, and interaction with its host are necessary. For that reason, several approaches have been performed using both classical genetic, cell biology, and biochemistry and the modern, hol...

Plant pathogenic fungi cause important yield losses in crops. In order to develop efficient and environment-friendly crop protection strategies, molecular studies of the fungal biological cycle and interaction with its host are necessary. For that reason, several approaches have been made using both classical genetic, cell biology and biochemistry and modern, holistic and high-throughput, omic ...

Proteomics techniques are continuously being developed to further understanding of biology and disease. Many of the pathways that are relevant to disease mechanisms rely on the identification of post-translational modifications (PTMs) such as phosphorylation, acetylation, and glycosylation. Much attention has also been focused on oxidative PTMs which include protein carbonyls, protein nitration...

The analysis of secreted proteins represents a challenge for current proteomics techniques. Proteins are usually secreted at low concentrations in the culture media, which makes their recovery difficult. In addition, culture media are rich in salts and other compounds interfering with most proteomics techniques, which makes selective precipitation of proteins almost mandatory for a correct subs...