The effects of some factors on the preparation and regeneration of protoplasts from the taxol-producing fungus Nodulisporium sylviforme were discussed in this paper, including combination of various enzymes, digesting time and temperature, and pH value, osmotic stabilizers, pretreation, culture medium and culture method. Under the condition of the mixed enzymes in osmotic pressure stabilizer (0...

Protoplasts from four different species of Streptomyces regenerated cells efficiently in hypertonic soft agar medium overlaid on partially dehydrated regeneration medium. The efficiencies of regeneration were strongly dependent upon the incubation temperatures for cell growth and for protoplast regeneration. Cell growth temperatures (before protoplast formation) required for efficient protoplas...

Protoplast wet densities (1.315 to 1.400 g/ml), determined by buoyant density sedimentation in Metrizamide gradients, were correlated inversely with the protoplast water contents (26.4 to 55.0 g of water/100 g of wet protoplast) of nine diverse types of pure lysozyme-sensitive dormant bacterial spores. The correlation equation provided a precise method for obtaining the protoplast water content...

Scented rose species are very important in the genus Rosa due to their use in highly-prized essential oil production and as garden roses. The fragrance of the rose flower (attar) captured by extraction is one of the most valuable flavor and fragrance products. A comprehensive study of the papers published in the field of in vitro culture and biotechnology of these species, particularly Damask r...

Protoplast culture and transformation technology offer a novel method for developing new plant varieties. Nonetheless, the effective preparation of protoplasts specific to celery has yet be achieved. This study utilized seedling leaves as primary materials examine key factors influencing protoplast isolation. The aim was prepare leaf with high yield quality subsequently conduct transient gene e...

one of the important technical obstacles in the study of many filamentous fungi is the development of efficient transformation system. transformation of filamentous fungi is difficult because they have a cell wall and for most frequently used approaches preparing of protoplast is necessary. protoplast preparation is batch-dependent and often frustrating work. in this study, a modified method wa...