The effect of repeated oral administration of ATP on purine transport and metabolism was investigated in rats. An increased ability of the gut to capture intraluminal purine nucleosides and to export ATP and nucleosides toward portal bloodstream was observed in rats after 30 days of treatment with 5 mg/kg/day ATP. This was accompanied in erythrocytes by an increased transport of adenosine rapid...

In brief: Metabolite signatures were compared between children with normal weight, obesity, and both obesity T2DM, by measuring 273 analytes in fasting plasma a 24-hour urine sample. Twenty-two metabolites uniquely associated T2DM. Adolescents T2DM have altered purine nucleotide metabolism, betaine oxidative branched-chain amino acids (BCAAs) their catabolites.

Several aspects of purine metabolism were studied in peripheral blood mononuclear cells and fibroblasts from a patient with purine nucleoside phosphorylase deficiency and compared to cells from normal controls. Intact cells were incubated with radioactive purine bases and all purine metabolites were extracted and analyzed. Incubation of purine nucleoside phosphorylase-deficient cells with [3H]h...

ducing carcinostasis, with tumor cells resistant to thio guanine, has been suggested (9). The aand @9-anomers of 2'-deoxythioguanosine (TGDR) have been shown to be active against ascites tumors having either of two types of resistance to thioguanine (10). The kinases necessary for conversion of these nucleosides to nucleotides were apparently present in the tumor cell lines examined. How ever, ...