Potato virus Y (PVY) coat protein gene has been cloned from an Indian isolate of PVY. The coat protein gene from this isolate showed 93.2% nucleic acid sequence homology and more than 94% amino acid sequence homology compared to an American isolate. The coat protein gene has been sub-cloned into plant expression vector pBINPLUS and tobacco leaf discs were transformed using Agrobacterium tumefac...

Potato virus Y (PVY) as one of the serious diseases on tobacco (Nicotiana tabacum L.), reduces leaf yield and its quality. Use of re-sistant genotypes is being considered as the most effective, economical and a safe way to reduce damages of the virus. In the present study, the response of 90 oriental and semi-oriental tobacco genotypes was evaluated against PVY using randomized complete block d...

The stability of hybrid plant viruses that might arise by recombination in transgenic plants was examined using hybrid viruses derived from the viral expression vectors potato virus X (PVX) and tobacco rattle virus (TRV). The potato virus Y (PVY) NIb and HCPro open reading frames (ORFs) were introduced into PVX to generate PVX-NIb and PVX-HCPro, while the PVY NIb ORF was introduced into a vecto...

A full-length cDNA clone of the potato virus Y (PVY) genome was obtained after cloning difficulties in Escherichia coli were overcome. These difficulties were mainly due to the expression of the CI gene from upstream prokaryotic promoter-like elements within the PVY genome. To overcome this problem, PVY cDNA was maintained in two subclones which were ligated before infection. A plasmid in which...

Potato virus Y (PVY) is an important plant pathogen with a wide host range that includes, among others, potato, tobacco, tomato and pepper. The coat protein (CP) of PVY has been commonly used in phylogenetic studies for strain classification. In this study, we used a pool of 292 CP sequences from isolates collected worldwide. After detecting and removing recombinant sequences, we applied Bayesi...

Potyviruses are one of the most destructive viral pathogens of Solanaceae plants. In Capsicum annuum landrace CM334, a broad-spectrum gene, Pvr4 is known to be involved in resistance against multiple potyviruses, including Pepper mottle virus (PepMoV), Pepper severe mosaic virus (PepSMV), and Potato virus Y (PVY). However, a potyvirus avirulence factor against Pvr4 has not been identified. To i...

Perception of pathogens by host pattern recognition receptors (PRRs) or R proteins is a prerequisite to promote successful immune responses. The Hsp70/Hsp90 organizing protein Hop/Sti1, a multifunctional cochaperone, has been implicated in the maturation of a receptor-like kinase (RLK) necessary for chitin sensing. However, it remains unknown whether Hop/Sti1 is generally participating in PRR g...

Risk-assessment studies of virus-resistant transgenic plants (VRTPs) focussing on recombination of a plant virus with a transgenic sequence of a different virus should include a comparison of recombination frequencies between viruses in double-infected non-transgenic plants with those observed in singly infected transgenic plants to estimate recombination incidence in VRTPs. In this study, the ...

Potato virus Y (PVY) is an important plant pathogen causing considerable economic loss to potato production. Knowledge of the population genetic structure and evolutionary biology of the pathogen, particularly at a transnational scale, is limited but vital in developing sustainable management schemes. In this study, the population genetic structure and molecular evolution of PVY were studied us...

PeSMV was originally isolated in Argentina from diseased Capsicum annuum plants and classed as a possible member of the potyvirus group on the basis of morphological, biological, and immunological evidence (Feldman and Gracia, 1977). Although information concerning its economic significance is still imprecise, the virus is present in most pepperproducing areas and yields are drastically reduced...