The objective of this study was to investigate the impact of long-term pyrene exposure on molting and reproduction in the model estuarine invertebrate, the grass shrimp (Palaemonetes pugio). Grass shrimp were exposed to measured concentrations of 5.1, 15.0, and 63. 4 ppb (microg/L) pyrene for 6 weeks, during which time we determined molting and survivorship. At the end of the exposure, we immed...

SUMMARY Benzo(a)pyrene and five isomenic phenols of benzo (a)pynene were tested for carcinogenicity by the topical application of 0.4 @moIe of each compound to the backs of C57BL/6J mice once every 2 weeks for 60 weeks. 1-, 3-, and 12-hydroxybenzo(a)pyrene were noncarcinogenic , 11-hy droxybenzo(a)pyrene was weakly carcinogenic, and 2-hy droxybenzo(a)pyrene and benzo(a)pyrene were highly car ci...

The binding of [3H]benzo(a)pyrene to proteins of rat liver cytosol, the nuclear uptake of cytosolic protein-bound [3H]-benzo(a)pyrene, and the subsequent nuclear metabolism of the polycyclic hydrocarbon were investigated. The binding of [3H]benzo(a)pyrene to cytosol had a saturable high affinity component with a Kd of 2.54 nM and a capacity of 530 fmol/mg protein. Specific binding of [3H]benzo(...

In vitro studies have previously indicated that benzo(a)pyrene distributes primarily into the plasma lipoprotein fraction when incubated with whole plasma. Hydroxylated metabolites of benzo(a)pyrene distribute increasingly into the albumin fraction as the degree of metabolite hydroxylation increases. This report assesses the influence of plasma lipoproteins and albumin as carriers for benzo(a)p...

Iron-pyrene cluster anions, [Fe(m)(pyrene)(n)](-) (m = 1-2, n = 1-2) were studied in the gas phase by photoelectron spectroscopy, resulting in the determination of their electron affinity and vertical detachment energy values. Density functional theory calculations were also conducted, providing the structures and spin multiplicities of the neutral clusters and their anions as well as their res...

The environment is heavily populated with polycyclic aromatic hydrocarbons (PAHs), which are dangerous to human health. Degradation and cleaning of PAH chemicals from water soil regions crucial due their chemical biological impacts persistent nature. In this study, we found that a very efficient bacterial consortium A-LOBP-19A+LOP-9 (99.62%) for benzo[a]pyrene up 1000ppm B-LOP-9 +GWP-2 (93.8%) ...

The metabolism of benzo(a)pyrene has been investigated in cultured normal human bronchus, colon, duodenum, and esophagus obtained from the same patient. The highest total metabolism was found in bronchus and duodenum, while the highest mean binding level was observed in the bronchus followed, in order, by the esophagus, duodenum, and trans verse colon. A 30-fold interindividual variation in the...

The tumorigenic activities of benzo(e)pyrene and several of its derivatives were determined in two mouse tumor models. Newborn Swiss-Webster mice were given i.p. injections of 0.4, 0.8, and 1.6 mumol of compound on the first, eighth, and 15th day of life, respectively. When the mice were 62 to 66 weeks old, the experiment was terminated by killing the animals. Benzo(e)pyrene, trans-4,5-dihydrox...

OBJECTIVES We studied the uptake of polycyclic aromatic hydrocarbons (PAH) in nurses who apply ointments containing coal tar to patients and investigated the effectiveness of skin protection methods. METHODS We determined gas-phase PAH on XAD-2 and particle-associated PAH on filters. We also used pads to determine PAH on the skin. Pyrene and benzo(a)pyrene were analyzed by gas chromatography/...

The exposure of Estonian cokery workers to polynuclear aromatic hydrocarbons at an oil shale processing plant was assessed by occupational hygiene and biomonitoring measurements. To assess the external dose of exposure to polynuclear aromatic hydrocarbons, pyrene and benzo[a]pyrene concentrations were measured from the breathing zone of workers during a workshift. Skin contamination with pyrene...