Ultraviolet (UV) irradiation is advantageous as a sterilization technique in the biopharmaceutical industry since it is capable of targeting non-enveloped viruses that are typically challenging to destroy, as well as smaller viruses that can be difficult to remove via conventional separation techniques. In this work, we investigated the influence of oxygen in the media during UV irradiation and...

The general nature of the biological transamination reaction (1) has been amply demonstrated in the past few years (2-7). It has been suggest’ed (8) that the coenzyme, via alternate interconversions into pyridoxal and pyridoxamine phosphates, functions as t’he shuttle of the amino groups in these reactions. If this mechanism is valid, it t’hen follows that enzymatic transaminations between the ...

Pyridoxamine (PM) is a promising drug candidate for treatment of diabetic nephropathy. The therapeutic effect of PM has been demonstrated in multiple animal models of diabetes and in phase II clinical trials. However, the mechanism of PM therapeutic action is poorly understood. One potential mechanism is scavenging of pathogenic reactive carbonyl species (RCS) found to be elevated in diabetes. ...

An alternatively minimizing covariant matrix error (AMCME) algorithm, newly proposed by the present authors, was applied to the simultaneous fluorometric determination of pyridoxal, pyridoxamine and 4-pyridoxic acid without loss of sensitivity. The experimental results illustrate that the profiles of spectra and concentration can be accurately resolved using the AMCME algorithm with a high sens...

A series of dendritic gadolinium complexes based on dendrimers with 1,4,7,10-tetraazacyclododecane as the core were synthesized. Both diethylenetriaminepentaacetic acid and pyridoxamine were incorporated to the amine groups on the surface to give the dendritic ligands. These dendritic ligands were then reacted with gadolinium chloride to yield the corresponding dendritic gadolinium complexes. T...

A procedure is described for the purification of CDP-Dglucose oxidoreductase which catalyzes formation of CDP-4keto-6-deoxy-D-glucose and for enzymes El and ES which catalyze the further reduction to CDP-4-keto-3,6-dideoxy-Dglucose. All three enzymes have been purified to homogeneity as judged by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. CDP-D-glucose oxidore...