This study employed the response surface methodology to optimize extraction conditions for recovering vitamins D2 and K1 from green leafy vegetables using ultrasonication-assisted extraction. The vitamin content was determined an Accucore C18 column a UPLC-Q-ToF/MS method. An RSM-I-Optimal design used designing experiment find best combination of solvent level (mL), sonication time (min), frequ...

A study was performed to elucidate opiate-induced metabolic changes in murine brain. The EI MS, EI MS/MS, and PCI capabilities of the Agilent 7200 Series GC/Q-TOF MS, in combination with Agilent MassHunter Software tools, enabled a very flexible and comprehensive workflow for identifying metabolomic differences. The workflow was used to distinguish between morphine-sensitive and resistant murin...

Curcuma, a genus of rhizomatous herbaceous species, has been used as a spice, traditional medicine, and natural dye. In this study, the metabolite profile of Curcuma extracts was determined using gas chromatography-time of flight mass spectrometry (GC/TOF MS) and ultrahigh-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF MS) to characterize differences b...

The use of Accurate-Mass Q-TOF LC/MS and MS/MS, in both positive and negative electrospray ionization (ESI) modes, was evaluated for the identification of sucralose in water. Response and fragmentation pathways were investigated. Sucralose responded well using Q-TOF LC/MS in either the positive or negative ion ESI modes. The overall signal intensity obtained in positive ion mode was approximate...

Objective This study aimed to explore the immunoregulatory effect of flavonoids of blueberry (Vaccinium corymbosum L.) leaves (FBL). Methods The flavonoids of blueberry leaves were prepared with 70% ethanol and were identified by ultraperformance liquid chromatography/quadrupole-time-of-flight mass spectrometry (UPLC/Q-Tof-MS). The immunoregulatory effect and possible regulatory mechanisms of...

Hyperlipidemia is considered to be a high lipid level in blood, can induce metabolic disorders and dysfunctions of the body, and results in some severe complications. Therefore, hunting for some metabolite markers and clarifying the metabolic pathways in vivo will be an important strategy in the treatment and prevention of hyperlipidemia. In this study, a rat model of hyperlipidemia was constru...

Honey adulteration with sugar syrups is a widespread problem. Several types of syrups have been used in honey adulteration, and there is no available method that can simultaneously detect all of these adulterants. In this study, we generated a small-scale database containing the specific chromatographic and mass spectrometry information on sugar syrup markers and developed a simple, rapid, and ...

With the implementation of strict regulations on the maximum allowable residue levels (MRLs) of pesticides in food matrixes worldwide, sensitive, reliable, and high-throughput analytical methodologies are urgently required for rapid screening and monitoring of the level of pesticide residues in foodstuffs. In this application note, we have detailed a method on QuEChERS extraction and cleanup pr...