Quantitative Real-Time Polymerase Chain Reaction, better known as qPCR, is the most sensitive and specific technique we have for the detection of nucleic acids. Even though it has been around for more than 30 years and is preferred in research applications, it has yet to win broad acceptance in routine practice. This requires a means to unambiguously assess the performance of specific qPCR anal...

In studies monitoring disease progression and therapeutic response, it is essential that the method used for hepatitis C virus (HCV) quantification not be influenced by genotypic variability. The branched DNA assay provides a reliable method for the quantification of HCV RNA. A modified set of oligonucleotide probes for the branched DNA assay was developed to enhance the efficiency of binding t...

The paper describes a simple gas chromatographic method for quantification of ethanol in distillates of chocolate shell pralines and fillings. The samples were prepared in two steps. The first step consisted of ethanol distillation from the product and the second involved capillary gas chromatography of 10% v/v distillate with expected ethanol content between 0.06% and 2.5% w/w. Quantification ...

Dextran sulfate is semi-synthetic, polydisperse sulfated polysaccharide with important applications in clinical practice, in the manufacturing of plasma derived protein therapeutics and in biomedical research. The sensitive detection of dextran sulfate is relevant to preclinical and clinical drug development projects, the quality control of pharmaceutic formulations, and the process control in ...

abstract polycyclic aromatic hydrocarbons (pahs) are classified as persistent and carcinogenic organic pollutants. pahs contamination has been reported in water. many of relevant regulatory bodies such as eu and epa have regulated the limit levels for pahs in drinking water. in this study, 13 priority polycyclic aromatic hydrocarbons (pahs) were determined in tap water samples of tehran and wat...

BACKGROUND Propionic acid is a widely used preservative and has been mainly formed by artificial synthesis or fermentation. In the case of natural products, the presence of propionic acid is viewed as a sign that an additive has been introduced for antimicrobial effects. METHODS In this work, the propionic acid that occurs in Scutellaria baicalensis roots was studied. A quantification method ...

In a previous paper, a computational method was presented for determining statistically rigorous limits of detection and quantification. The main purpose of this study is to evaluate similar but less computationally complex methods. These “approximate” methods use data at multiple spiking concentrations, are iterative, can be derived from either prediction intervals or statistical tolerance int...

The resolving power of high-resolution transmission electron microscopes is characterized by the information limit, which reflects the size of the smallest object detail observable with a particular instrument. We introduce a highly accurate measurement method for the information limit, which is suitable for modern aberration-corrected electron microscopes. An experimental comparison with the t...

OBJECTIVE To prospectively compare technical success rate and reliable measurements of virtual touch quantification (VTQ) elastography and elastography point quantification (ElastPQ), and to correlate liver stiffness (LS) measurements obtained by the two elastography techniques. MATERIALS AND METHODS Our study included 85 patients, 80 of whom were previously diagnosed with chronic liver disea...