1. Methods of homogenizing suspensions of washed mammalian spermatozoa were studied. The most useful methods were those using sonication and those using a French press. 2. Hexokinase, phosphofructokinase, glucose phosphate isomerase and adenosine triphosphatase activities in ram, bull and boar spermatozoa were investigated by using these two homogenization methods. Glucose phosphate isomerase, ...

In the procedure described, demembranated ram sperm models were treated to decondense their nuclei, and the movement of their sperm tails was then reactivated. The duration of movement was sustained for more than 30 min, with a tail-wave frequency of up to 14 Hz. Decondensed sperm models adhered to each other and to the slide; but adherence was prevented by addition of an anti-agglutination fac...

Background: Seminal plasma of mammals is a complex fluid, which serves as a carrier for the spermatozoa on their journey from the male testes to their target, the oocyte. Seminal plasma contains a variety of biochemical components, some of which are relatively specific for the regulation of sperm function. The objective of this study was to investigate enzyme activities aspartate-amino-transfer...

In this study, certain enzymes in ram semen involved in reactive oxygen species elimination and their changes during the cryopreservation process were characterized in order to investigate the hypothesis that the antioxidant defense system is involved in the maintenance of frozen sperm quality. Glutathione reductase (GR), glutathione peroxidase (GPx), and superoxide dismutase (SOD) activities w...

The effect of seminal plasma, as well as some seminal plasma fractions, on the heterogeneity and viability of frozen-thawed ram spermatozoa was studied. Fresh and frozen sperm samples were simultaneously assessed by a routine semen-quality assay and centrifugal countercurrent distribution (CCCD) in an aqueous two-phase system analysis. All samples used for this study were obtained from Salz ram...

The accurate measurement of semen fertilizing potential is of great importance in determining the acceptability of processed semen for breeding purposes. A good sperm preparation technique results in a sample with high viability and motility and also takes into account other parameters such as the capacitation and apoptotic state which could compromise the ability to fertilize an oocyte. In thi...

The role of seminal plasma (SP) in mammalian sperm function remains largely a matter of speculation as both inhibitory and stimulating effects have been found. Specific components of SP, particularly proteins, are adsorbed onto the surface of ejaculated sperm as they pass through the male and female reproductive tracts. These sperm coating components seem to have the important function of maint...

background: this study was conducted to evaluate the effect of melatonin on semen quality and quantity changes and increase of reproductive efficiency in lori-bakhtiari ram in the non-reproductive season. objectives: the purpose of this study was to investigate the effect of melatonin on testicular size and semen qualitative and quantitative changes in the non-breeding season is lori-bakhtiari ...

The objective of this research was to evaluate the effect sperm on chromatin stability and its relationship with membrane integrity structural – physiological rate fertilization female sheep. Ejaculates (2 × 109 sperm·mL-1) 70% motility were collected using an artificial vagina (n=5, 2 years old. For this, each ram served fifteen sheep (n=75), generating thus five different Groups (A, B, C, D, ...