Methods for identifying isolates of various pathogenic bacteria by DNA fingerprinting with random primers (RAPD) have been described recently. In these methods many primers are screened and the primers that generate the most informative DNA pattern are selected. A new strategy that simplifies the primer selection process for RAPD fingerprinting has been developed in our laboratory. In this appr...

Over the last two decades, DNA fingerprinting have been increasingly used to characterize germplasm of different organisms including crop plants, which was otherwise difficult with morphological and or isozyme markers because of paucity of genetic information at phenotypic level. In the present study, two hybrid samples of sorghum (Sorghum bicolor L.) received from Federal Seed Certification an...

leaf blotch disease of barley, caused by rhynchosporium secalis is a major disease of barley in iran. its worldwide occurrence and economic importance in barley production has motivated studies on the population genetic structure of this pathogen. random amplified polymorphic dna (rapd) method was utilized to investigate the genetic diversity of populations of r. secalis isolated from barley le...

Genetic variability and relationships among ten rice varieties were studied by using 20 decamer random primers and SDSPAGE methods. Out of 22, 20 Random Amplified Polymorphic DNA (RAPD) primers revealed polymorphism, while the remaining 2 primers showed no reaction. The primers produced a total of 109 bands, of which 62 (56.88%) were polymorphic. The number of polymorphic fragments for each pri...

genetic diversity of 36 genotypes of dwarf and semi-dwarf apple rootstocks along with seven iranian dwarf rootstocks were investigated by use of rapd dna markers. a set of 100 random primers was utilized in pcr reaction from which 10 primers resulted in good amplification with high polymorphism. among primers, 10 selected rapd primers produced 160 bands, in which, 136 were polymorphic. cluster ...

The Ayurvedic medical system, which uses herbal preparations for its traditional remedies, has been used in India since ancient times. One of the powerful immunomodulators is Tinospora cordifolia, also known as AMRITA, credited to this miracle medication giving user youth, longevity, and vigour. RAPD widely successfully identify several species medicinally significant plant. need research into ...

A study involving the use of random amplified polymorphic DNA (RAPD) was conducted to evaluate genetic polymorphism and relatedness within and among four chicken breeds: Araucona, Rhode Island Red, White Leghorn, and White Plymouth Rock, and two turkey populations, a long-term randombred and a commercial strain. A total of 60 random primers were used in the RAPD analyses. Forty-two of the 60 pr...

The production of informative random amplified polymorphic DNA (RAPD) markers using PCR and a single primer is often accompanied by the generation of artifactual (noninformative) bands as well. When RAPD data are used to compute genetic similarity coefficients, these artifacts (false positives, false negatives, or both) can cause large biases in the numerical values of the coefficients. As a re...