An Actinomycete, strain K-128, which was isolated from soil, formed a transfer product when the strain was cultured in a medium containing both stevioside and curdlan. The transfer product in culture broth was separated by DIAION HP-20 column and TOYOPEARL HW-40F column chromatographies. From structural studies, the transfer product was identified as C13-O-beta-6(2)-beta-glucosylsophorosyl-C19-...

In the course of our investigation for the antibiotic-producing actinomycetes. Thirty-two actinomycete strains were isolated from Thai hot spring sediment. The generic identities of these isolates were determined by using a procedure that combined morphological, chemotaxonomic and 16S rDNA sequence-based phylogenetic analyses. Actinomycetes belonging to a total of 4 genera were identified. They...

A flavocytochrome protein was isolated from the actinomycete Streptomyces antibioticus. The purified protein contained protoheme and FAD, and its M(r) was estimated to be 52000. The absorption spectra in its resting oxidized, dithionite-reduced, carbon monoxide-bound, and oxygenated (O(2)-bound) forms were characteristic of those of flavohemoglobin (Fhb). The N-terminal amino acid sequence show...

Actinomycetes were isolated from eight samples of two different habitats, i.e., garden soil and cultivated field soil. Isolation of actinomycetes was carried out on soil extract agar medium using serial dilution method and forty seven isolates were obtained. All these actinomycetes were then assessed for their antifungal activity against two fungal pathogens i.e., Candida albicans and Aspergill...

Actinomycetes, filamentous actinobacteria found in numerous ecosystems around the globe, produce a wide range of clinically useful natural products (NP). In natural environments, actinomycetes live in dynamic communities where environmental cues and ecological interactions likely influence NP biosynthesis. Our current understating of these cues, and the ecological roles of NP, is in its infancy...

Several antibiotics were tested against a range of actinomycetes, bacteria and fungi representing types found in soil. From these tests four antibiotics, nystatin (50 pg./ml.), actidione (50 pg./ml.), polymyxin B sulphate (5.0 pg./ml.) and sodium penicillin (1.0 ,ug./ml.), were selected for incorporation into a starch +casein medium to achieve selective growth of actinomycetes on soil dilution ...

Soil microbes represent an important source of biologically active compounds. These molecules present original and unexpected structure and are selective inhibitors of their molecular targets. At Biosearch Italia, discovery of new bioactive molecules is mostly carried out through the exploitation of a proprietary strain collection of over 50000 strains, mostly unusual genera of actinomycetes an...

BACKGROUND AND PURPOSE Actinomycetes have been discovered as source of antifungal compounds that are currently in clinical use. Invasive aspergillosis (IA) due to Aspergillus fumigatus has been identified as individual drug-resistant Aspergillus spp. to be an emerging pathogen opportunities a global scale. This paper described the antifungal activity of one terrestrial actinomycete against the ...