Cells of the mucosal mast cell line, RBL-2H3, are normally stimulated to degranulate after aggregation of high affinity receptors for IgE (Fc epsilon RI) by soluble cross-linking ligands. This cellular degranulation process requires sustained elevation of cytoplasmic Ca2+. In this study, we investigated the response of RBL-2H3 cells to 6-micron beads coated with IgE-specific ligands. These liga...

Although mast cell secretion has been intensively studied because of its pivotal role in allergic reactions and its advantages as a physiologic model, the molecular composition of the secretory machine is virtually unknown. In view of the guanine-nucleotide dependency of mast cell exocytosis and the participation of Rab3 proteins in synaptic vesicle release, we hypothesized that a Rab3 isoform ...

The problem of formulating the chiral fermion field on space-time lattices has been discussed from the viewpoint of funtional integrals [1]. I argued that the functional integral measure provided by any single lattice ( hypercubical lattice or random lattice ) is not sufficent to yield fermionic functional integrals to agree with the continuum field theory. My proposal of using an ensemble of r...

The IL-8 (CXCL8) receptors CXCR1 and CXCR2 couple to Gαi to induce leukocyte recruitment and activation at sites of inflammation. We recently showed that CXCR1 couples predominantly to the G protein-coupled receptor kinase (GRK)2, whereas CXCR2 interacts with GRK6 to regulate cellular responses. In addition to G protein-coupled receptors, GRKs displayed a more diverse protein/protein interactio...

The problem of formulating the chiral fermion field on space-time lattices has been discussed from the viewpoint of funtional integrals [1]. I argued that the functional integral measure provided by any single lattice ( hypercubical lattice or random lattice ) is not sufficent to yield fermionic functional integrals to agree with the continuum field theory. My proposal of using an ensemble of r...

The rhizomes of Acorus gramineus have frequently been used in traditional medicine mainly for sedation as well as enhancing brain function. In this study, the anti-allergic activity of A. gramineus was investigated. The 70% ethanol extract of the rhizomes of A. gramineus was found to inhibit the allergic response against 5-lipoxygenase (5-LOX)-catalyzed leukotriene (LT) production from rat baso...

The high affinity receptor for IgE on rat basophilic leukemia (RBL) cells mediates antigen-triggered cellular degranulation. Polyethylene glycol-induced membrane fusion methods were used to introduce exogenous IgE receptors into living RBL cells, and these were tested for normal activities. In cell-cell fusion experiments, RBL cells with fluorescein-labeled rat IgE bound to receptors and contai...

This review was the typical research-based learning (RBL) approach in conducting surface interpretation higher education. We believe RBL is applied general sciences and may be used Alquran room as a tool to understand specific lesson contexts. Therefore, we have reviewed many data sources from different databases searching electronically. The are publications for academic works such journals, b...

Three common food colorants (tartrazine TT, allura red AR, and sunset yellow SY) were simultaneously quantified in powdered soft drinks by employing a combination of absorbance spectra-pH data matrices and multivariate processing of the generated second-order data. Parallel factor analysis PARAFAC and bilinear least squares/residual bilinearization BLLS/RBL were applied for deconvolution of tri...

Mast cells play a critical role in immunoglobulin E (IgE)-mediated allergic diseases, and the degranulation of mast cells is important in the pathogenesis of these diseases. A disturbance of the intestinal microflora, especially of endogenous lactic acid bacteria, might be a contributing factor for IgE-mediated allergic diseases. Additional knowledge regarding the interaction of human intestina...