keywords: directed protein immobilization, recombinant antibody fragment

Background: The advent of recombinant DNA technology has facilitated heterologous expression of proteins from various sources in different host systems including Escherichia coli. If a plant virus coat protein is expressed in the bacterium it can be used as the antigen for antibody preparation. Such a recombinant antigen preparation can be particularly useful where equipment such as ultracentri...

Background: Erythropoietin, as a principal hormone promotes red blood cell production in bone marrow. Varieties of erythropoietin biosimilar are being produced by recombinant DNA technology in cell cultures. The detection or quantification of these molecules are being performed by diff erent methods which some of theme such as Western blot and enzymelinked immunosorbent assay (ELISA) require sp...

objective: escherichia coli o157:h7 is a gram-negative rod-shaped bacterium. e coli o157:h7 is an enterohemorrhagic (ehec) strain of the bacterium escherichia coli and a cause of foodborne illness. infection often leads to bloody diarrhea by producing a toxin called shiga toxin, which damages the intestines, and occasionally leads to kidney failure, especially in young children and elderly peop...

background   and   objectives:    the   toxin   co-regulated   pilus   a   (tcpa)   has   been   described   as   a   criti- cal  pathogenicity  factor  of   vibrio  cholerae .  tcpa  is  a  candidate  for  making  subunit  vaccine  against  chol- era.  the  aim  of  this  study  was  to  produce  a  candidate  vaccine  by  expressing  recombinant  tcpa  in   e.  coli . materials and methods: i...

antibodies provide a suitable tool in fundamental research and their high affinity and specificity make them invaluable for diagnostic and therapeutic applications. a promising alternative to conventional antibodies are the heavy chain antibodies (vhh) of camelidae having short length, high solubility and stability are preferred to other antibody derivatives. in this study, our goal was product...

Background: Hepatitis B is an important infectious disease.  Since several years ago, mass vaccination against this viral infection has become as part of routine vaccination schedule of Iran.  However, some healthy neonates, children and adults fail to generate a protective antibody response after vaccination.Objectives: To investigate distribution of HLA class-I and class-II antigens in health...