نتایج جستجو برای: recombinant core

تعداد نتایج: 324308  

2014
Ting Wang Zhi P. Cai Xiao Q. Gu Hong Y. Ma Ya M. Du Kun Huang Josef Voglmeir Li Liu

Peptide-N4-(N-acetyl-β-glucosaminyl) asparagine amidases [PNGases (peptide N-glycosidases), N-glycanases, EC 3.5.1.52] are essential tools in the release of N-glycans from glycoproteins. We hereby report the discovery and characterization of a novel bacterial N-glycanase from Terriglobus roseus with an extremely low pH optimum of 2.6, and annotated it therefore as PNGase H+. The gene of PNGase ...

Journal: :Gene 2012
Gregory J Bedwell Francis D Appling Susan J Anderson David A Schneider

Transcription of ribosomal DNA by RNA polymerase I is a central feature of eukaryotic ribosome biogenesis. Since ribosome synthesis is closely linked to cell proliferation, there is a need to define the molecular mechanisms that control transcription by RNA polymerase I. To fully define the factors that control RNA polymerase I activity, biochemical analyses using purified transcription factors...

Journal: :Methods in enzymology 2004
Pamela N Dyer Raji S Edayathumangalam Cindy L White Yunhe Bao Srinivas Chakravarthy Uma M Muthurajan Karolin Luger

The ability to prepare nucleosome core particles (NCPs), or nucleosomal arrays, from recombinant histone proteins and defined-sequence DNA has become a requirement in many projects that address the role of histone modifications, histone variants, or histone mutations in nucleosome and chromatin structure. This approach offers many advantages, such as the ability to combine histone variants and ...

Journal: :Acta Crystallographica Section A Foundations of Crystallography 2005

Journal: :Journal of virology 1999
P S Polacino V Stallard J E Klaniecki S Pennathur D C Montefiori A J Langlois B A Richardson W R Morton R E Benveniste S L Hu

We previously showed that envelope (gp160)-based vaccines, used in a live recombinant virus priming and subunit protein boosting regimen, protected macaques against intravenous and intrarectal challenges with the homologous simian immunodeficiency virus SIVmne clone E11S. However, the breadth of protection appears to be limited, since the vaccines were only partially effective against intraveno...

2015
Emi Ito Ritsuko Oka Takeo Ishii Hiroaki Korekane Ayako Kurimoto Yasuhiko Kizuka Shinobu Kitazume Shigeru Ariki Motoko Takahashi Yoshio Kuroki Kozui Kida Naoyuki Taniguchi

In order to verify the protein enriched from pooled human sera to be a lung-specific protein surfactant protein-D (SP-D), we performed peptide mass fingerprinting (PMF)-based protein identification. MASCOT search results of the obtained PMF unequivocally demonstrated that it is identical to human SP-D. Meanwhile, we performed MALDI-QIT-TOF mass spectrometry-based N-glycomic analysis of the reco...

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