L-2-methyltryptophan was prepared from L-serine and 2-methylindole catalyzed by recombinant Escherichia coli with tryptophan synthase activity. In the present research, we used tryptophan synthase (TSase) from recombinant Escherichia coli with pET28a-trpBA plasmid expressing tryptophan synthase from Escherichia coli k-12 MG1655 to produce 2-methyltryptophan, and optimized the conditions for enz...

purpose: in order to express human granulocyte-macrophage colony stimulating factor (hgm-csf) under heat shock. materials and methods: two expression plasmids were constructed based on pbc(sk) plasmid. the expression cassettes in the two plasmids are equipped with a 75 base pair fragment, derived from the pl promoter of the bacteriophage lambda (λ). the plasmids also contain a temperature muta...

Escherichia coli strain BL21 is one of the widely used bacterial hosts for high-level recombinant protein production and for other applications. Here, we present the complete genome sequence of a commercial version of the Escherichia coli BL21 strain.

background: as a drug target and an antigenic agent, hiv-1 protease (hiv-1 pr) is at the center of attention for designing anti-aids inhibitors and diagnostic tests. in previous studies, the production of the recombinant protease has been faced with several difficulties; therefore, the aims of this study were the easy production, purification of the soluble form of protease in e. coli and inves...

human insulin-like growth factor 1 (higf-1) consists of 70 amino acids in a single chain with three intermolecular disulfide bridges possessing valuable therapeutic effects. to date, numerous variants of specifically engineered higf-1 have been produced so as to improve higf-1 biological activity, stability and stronger binding to igf-1 receptor. mecasermin is one of the modified variants with ...

The study aims to produce Expansin protein isolated from a young tomato plant by using Escherichia coli which is used in recombinant production. Continuous culture the most common method grow cells for In study, K12 strain of E. was as production protein. LeExp1 gene plant. Since related found very small amounts plants, it has been reproduced PCR and made workable with this method. T17 vector (...

In two proof-of-concept studies, we established and validated the Bacterial Growth Inhibition Screen (BGIS), which explores recombinant protein toxicity in Escherichia coli as a largely overlooked alternative means for basic characterization of functional eukaryotic domains. By applying BGIS, identified an unrecognized RNA-interacting domain DEK oncoprotein (this study) successfully combined BG...

background the development of an effective subunit vaccine against brucellosis is a research area of intense. but optimization of recombinant proteins production in escherichia coli and content of endotoxins associated with final recombinant proteins are very important. objectives in the present study, expression and purification of brucella melitensis rhsp and rtf were optimized to reduce endo...

Background: Enterotoxigenic Escherichia coli (ETEC) strains are the major causes of diarrheal disease in humans and animals. Colonization factors and enterotoxins are the major virulence factors in ETEC pathogenesis. For the broad-spectrum protection against ETEC, one could focus on colonization factors and non-toxic heat labile as a vaccine candidate. Methods: A fusion protein is composed of a...

pneumococcal autolysins are enzymes involved in cell wall turnover and cellular division physiologically. they have been found to be involved in the pneumococcus pathogenesis. the aim of this study was to identify the autolytic activity of spr1754 as a novel protein of streptococcus pneumoniae . moreover, the binding of the recombinant protein to plasma proteins was also determined. the spr1754...