OBJECTIVE Anti-citrullinated protein antibodies (ACPAs) are characteristic of rheumatoid arthritis (RA). However, their presence years before the onset of clinical RA is perplexing. Although multiple putative citrullinated antigens have been identified, no studies have demonstrated the specific capacity of these antigens to initiate inflammatory arthritis. This study was undertaken to recapitul...

background: the advent of recombinant dna technology has facilitated heterologous expression of proteins from various sources in different host systems including escherichia coli. if a plant virus coat protein is expressed in the bacterium it can be used as the antigen for antibody preparation. such a recombinant antigen preparation can be particularly useful where equipment such as ultracentri...

Background: Targeting of CD20 antigen with monoclonal antibodies has become the mainstay in the treatment of non-Hodgkin's lymphomas and immunotherapeutic depletion of malignant B cells. Accessibility of antigen is one of the crucial factors in development of monoclonal antibodies against this antigen. One major problem in expression of full length CD20 is aggregation and misfolding. Therefore,...

background and objectives: toxoplasma gondii is an obligatory intracellular parasite which causes severe diseases in the fetus of pregnant women and immunocopmromised patients. serological tests based on recombinant protein are one of the main diagnosis methods for the detection of specific antibodies in serum samples. dense granule antigenic proteins derived from t. gondii (tggras) are potenti...

background: parasite proteases have important roles in cleavage of host proteins during the invasion of host tissues and participate in the parasite’s evasion from the host’s immune response. the aim of the present study was to estimate a metalloproteinase properties of taenia solium metacestode (tsmp) during host-parasite interactions, and evaluate its potential as a serodiagnostic antigen for...

Abstract The objective of this work was to compare different methods estimate heritability in 30 pigeon pea families from the F3, F4, F5, and F6 generations, for nine variables. experimental design a randomized complete block with three replicates 20 plants per plot. Broad-sense estimated by analysis variance ( A N O V A) [ h 2b-E(MS)], restricted maximum likelihood/best linear unbiased predict...

Background: Mycobacterium tuberculosis is the causative agent of tuberculosis (TB). Bacille Calmette-Guerin (BCG) vaccine, is not effective in adults, therefore, many efforts have been made to produce an effective adult TB vaccine. The aim of this study was to develop a new tuberculosis DNA vaccine candidate encoding a recombinant HspX-PPE44-EsxV fusion antigen of M. tuberculosis. Methods: ...

background: we have previously reported that a 31 kda protein was screened from the excretory-secretory (es) proteins of tichinella spiralis muscle larvae (ml) by immunoproteomics using early infection sera, and the gene encoding a 31 kda protein from t. spiralis was cloned and expressed in an e. coli expression system. in this study, the recombinant 31 kda antigens were used for detection of a...