With the advent of recombinant follicle stimulating hormone (FSH) and luteinizing hormone (LH), free from contamination with each other (Loumaye et al., 1995), it is possible to dissect the individual contributions of FSH and LH to the regulation of ovarian function and to define optimal gonadotrophin usage in controlled ovarian hyperstimulation (COH) (Hillier, 1994). Basic and clinical researc...

A 30-year-old man who presented with delayed puberty and infertility was found to have hypogonadism associated with an absence of circulating luteinizing hormone. The patient had a homozygous missense mutation in the gene that encodes the beta subunit of luteinizing hormone (Gly36Asp), a mutation that disrupted a vital cystine knot motif and abrogated the heterodimerization and secretion of lut...

Adenyl cyclase and cyclic 3’,5’-nucleotide phosphodiesterase were shown in homogenates of bovine corpora lutea. In order to determine the site of action of luteinizing hormone, the adenyl cyclase activity was assessed in the absence of phosphodiesterase activity. This was accomplished by using a high concentration of theophylline and short incubation periods. Under these conditions, luteinizing...

The presence of adenosine 3’,5’-monophosphate was demonstrated in bovine luteal tissue. The addition of luteinizing hormone to incubating slices of a bovine corpus luteum caused a rapid accumulation of this cyclic nucleotide which preceded the increase in progesterone synthesis. This effect appeared to be specific for luteinizing hormone. Puromycin did not inhibit this action of luteinizing hor...

Protein carboxymethylase, an enzyme capable of methylating proteins and polypeptides, was purified from bovine pituitary. The anterior pituitary hormones, luteinizing hormone, follicle-stimulating hormone, adrenocorticotropic hormone, growth hormone, thyroid-stimulating hormone, and prolactin, were found to be substrates for this enzyme. The posterior pituitary hormones, oxytocin and vasopressi...

The effect of graded exercise on the secretion of cortisol, testosterone, prolactin, growth hormone, thyroid stimulating hormone (TSH), luteinizing hormone and follicle stimulating hormone (FSH) is reported. While cortisol, prolactin, growth hormone and testosterone rise during the period of exercise, a rise in luteinizing hormone becomes evident only after rest. Levels of FSH and TSH remained ...